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Cloning and functional characterization of the early-lymphocyte-specific Pb99 gene

  1. Author:
    Sleckman, B. P.
    Khan, W. N.
    Xu, W. P.
    Bassing, C. H.
    Malynn, B. A.
    Copeland, N. G.
    Bardon, C. G.
    Breit, T. M.
    Davidson, L.
    Oltz, E. M.
    Jenkins, N. A.
    Berman, J. E.
    Alt, F. W.

  2. Author Address

    Alt FW Childrens Hosp, Howard Hughes Med Inst Longwood Ave Boston, MA 02115 USA Childrens Hosp, Howard Hughes Med Inst Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Genet Boston, MA 02115 USA Ctr Blood Res Boston, MA 02115 USA Univ Maryland, Ctr Med Biotechnol Baltimore, MD 21201 USA Univ Maryland, Dept Microbiol & Immunol Baltimore, MD 21201 USA Univ Maryland, Program Mol & Cell Biol Baltimore, MD 21201 USA NCI, Mammalian Genet Lab, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Molecular and Cellular Biology
    1. 20
    2. 12
    3. Pages: 4405-4410
  4. Type of Article: Article
  1. Abstract:

    The Pb99 gene is specifically expressed in pre-B cells and thymocytes and not in mature B and T cells or nonlymphoid tissues, implying that it may function in early lymphoid development. We have previously described the cloning of an incomplete cDNA for Pb99. Here we report the isolation of full-length cDNAs and genomic clones for the murine Pb99 gene and the mapping of its location to mouse chromosome 8. Sequence analyses of different Pb99 cDNA clones suggest that there may be at least three forms of the Pb99 protein generated by differential processing of the Pb99 transcript. The cDNA with the longest open reading frame encodes a putative protein that has seven hydrophobic domains similar to those of seven membrane-spanning proteins, such as the classical G protein-coupled receptors. To directly address the role of the Pb99 protein in lymphoid development, Pb99-deficient mice were generated by gene targeting, and lymphocyte development in these mice was analyzed. [References: 35]

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