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The axillary lymphoid organ is an external, experimentally accessible immune organ in the zebrafish

  1. Author:
    Castranova, Daniel [ORCID]
    Kenton, Madeleine I [ORCID]
    Kraus, Aurora [ORCID]
    Dell,Christopher [ORCID]
    Park, Jong S [ORCID]
    Venero Galanternik, Marina [ORCID]
    Park, Gilseung [ORCID]
    Lumbantobing, Daniel N [ORCID]
    Dye, Louis [ORCID]
    Marvel, Miranda [ORCID]
    Iben, James [ORCID]
    Taimatsu, Kiyohito [ORCID]
    Pham, Van [ORCID]
    Willms, Reegan J [ORCID]
    Blevens, Lucas [ORCID]
    Robertson, Tanner F [ORCID]
    Hou, Yiran [ORCID]
    Huttenlocher, Anna [ORCID]
    Foley, Edan [ORCID]
    Parenti, Lynne R [ORCID]
    Frazer, J Kimble [ORCID]
    Narayan,Kedar [ORCID]
    Weinstein, Brant M [ORCID]

  2. Author Address

    Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA., Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda, MD, USA., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research , Frederick, MD, USA., Section of Pediatric Hematology-Oncology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA., Division of Fishes, Department of Vertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA., Microscopy and Imaging Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD, USA., Molecular Genomics Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD, USA., Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada., Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA.,
    1. Year: 2025
    2. Date: Jun 02
    3. Epub Date: 2025 04 01
  2. Journal: The Journal of Experimental Medicine
    1. 222
    2. 6
    3. Pages: e20241435
  4. Type of Article: Article
  5. Article Number: e20241435
  1. Abstract:

    Lymph nodes and other secondary lymphoid organs play critical roles in immune surveillance and immune activation in mammals, but the deep internal locations of these organs make it challenging to image and study them in living animals. Here, we describe a previously uncharacterized external immune organ in the zebrafish ideally suited for studying immune cell dynamics in vivo, the axillary lymphoid organ (ALO). This small, translucent organ has an outer cortex teeming with immune cells, an inner medulla with a mesh-like network of fibroblastic reticular cells along which immune cells migrate, and a network of lymphatic vessels draining to a large adjacent lymph sac. Noninvasive high-resolution imaging of transgenically marked immune cells can be carried out in ALOs of living animals, which are readily accessible to external treatment. This newly discovered tissue provides a superb model for dynamic live imaging of immune cells and their interaction with pathogens and surrounding tissues, including blood and lymphatic vessels. This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

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External Sources

  1. View Full Text
  2. DOI: 10.1084/jem.20241435
  3. PMID: 40167600
  4. PMCID: PMC11960710
  5. PII : 277359

Library Notes

  1. Fiscal Year: FY2024-2025
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