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Immortalization of rat middle ear epithelial cells by adeno 12- SV40 hybrid virus

  1. Author:
    Ueyama, S.
    Jin, S. J.
    Rhim, J. S.
    Ueyama, T.
    Lim, D. J.

  2. Author Address

    House Ear Inst, Gonda Dept Cell & Mol Biol, 2100 W 3rd St, 5th Floor, Los Angeles, CA 90057 USA. House Ear Inst, Gonda Dept Cell & Mol Biol, Los Angeles, CA 90057 USA. NIDCD, Lab Cellular Biol, NIH, Rockville, MD USA. NCI, Lab Biochem Physiol, NIH, Frederick, MD 21701 USA. Lim DJ House Ear Inst, Gonda Dept Cell & Mol Biol, 2100 W 3rd St, 5th Floor, Los Angeles, CA 90057 USA.
    1. Year: 2001
  2. Journal: Annals of Otology Rhinology and Laryngology
    1. 110
    2. 2
    3. Pages: 132-141
  4. Type of Article: Article
  1. Abstract:

    Rat middle ear epithelial cells were infected with the adeno 12-SV40 hybrid virus. The cell line thus obtained displays features of primary cultured epithelial cells in both light microscopic and ultrastructural examinations. The immortalized cells have been in continuous proliferation for 40 passages and more than 17 months. Immunohistochemical analysis of the immortalized cells was positive for the SV40 T antigen and the tumor suppressor protein p53. The cells also stained positive for cytokeratin, an epithelial cell marker, and negative for vimentin. a fibroblast marker. These results, together with karyotype analysis, indicate that this cell line originated from rat middle ear epithelial cells and retains the characteristics of epithelial cells. This cell line will be useful for studying the normal cellular biology of middle ear epithelial cells, as well as the cellular and molecular mechanisms involved in the bacteria-middle ear epithelial cell interaction.

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