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A multicenter evaluation of assays for detection of SV40 DNA and results in masked mesothelioma specimens

  1. Author:
    Strickler, H. D.
    Goedert, J. J.
    Butel, J. S.
    Daniel, R.
    Freymuth, F.
    Gibbs, A.
    Griffiths, D. J.
    Jablons, D.
    Jasani, B.
    Jones, C.
    Lednicky, J. A.
    Miller, C. W.
    Radu, C.
    Richards, W. G.
    Shah, K. V.
    You, L.
    Corson, J. M.
    Gerwin, B.
    Harris, C.
    Sugarbaker, D. J.
    Egan, W.
    Lewis, A. M.
    Krause, P. R.
    Peden, K.
    Levine, A. S.
    Melnick, S.
    Cosentino, M.
    da Costa, M.
    Devairrakam, V.
    Ji, J.
    Palefsky, J.
    Rasmussen, L.
    Shea, K.
    Wacholder, S.
    Waters, D.
    Wright, T.

  2. Author Address

    Albert Einstein Coll Med, Dept Epidemiol & Social Med, 1300 Morris Pk Ave, Belfer Bldg 1308-B, Bronx, NY 10461 USA. Albert Einstein Coll Med, Dept Epidemiol & Social Med, Bronx, NY 10461 USA. NCI, Viral Epidemiol Branch, NIH, Bethesda, MD USA. Brigham & Womens Hosp, Div Thorac Surg, Boston, MA 02115 USA. Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. Johns Hopkins Univ, Dept Mol Microbiol & Immunol, Baltimore, MD USA. Deutsch Krebsforschungszentrum, D-6900 Heidelberg, Germany. Hosp Univ Caen, Lab Virol Humaine & Mol, Caen, France. Univ Wales, Coll Med, Dept Pathol, Cardiff, S Glam, Wales. Univ Coll London, Windeyer Inst Med Sci, Wohl Vir Ctr, London, England. Univ Calif San Francisco, Thorac Oncol Lab, San Francisco, CA 94143 USA. Univ Calif Los Angeles, Cedars Sinai Res Inst, Dept Med, Los Angeles, CA USA. Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA. NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. US FDA, Off Vaccines Res & Review, Ctr Biol Evaluat & Res, Bethesda, MD 20014 USA. NICHHD, NIH, Bethesda, MD 20892 USA. Univ Pittsburgh, Sch Med, Pittsburgh, PA USA. NCI, Div Canc Control & Populat Sci, NIH, Bethesda, MD 20892 USA. BBI Biotech Res Labs, Gaithersburg, MD USA. Res Triangle Inst, Rockville, MD USA. Sci Applicat Int Corp, Frederick, MD USA. NCI, Biostat Branch, NIH, Bethesda, MD 20892 USA. Columbia Univ, Coll Phys & Surg, Dept Pathol, New York, NY USA. Strickler HD Albert Einstein Coll Med, Dept Epidemiol & Social Med, 1300 Morris Pk Ave, Belfer Bldg 1308-B, Bronx, NY 10461 USA.
    1. Year: 2001
  2. Journal: Cancer Epidemiology Biomarkers & Prevention
    1. 10
    2. 5
    3. Pages: 523-532
  4. Type of Article: Article
  1. Abstract:

    This nine-laboratory multicenter investigation was designed to assess the sensitivity, specificity, and reproducibility of previously described assays for detection of SV40 DNA with three goals, i.e., (a) to compare methods for testing human tissues, (b) to examine the ability of these methods to detect SV40 in human mesotheliomas, and (c) to uncover assay differences that could explain conflicting findings in some past investigations. Each laboratory received, in a masked fashion, paired replicate DNA samples extracted from 25 fresh frozen mesotheliomas (50 samples) and one from each of 25 normal human lungs. Interspersed were masked positive (titrations of the SV40 genome) and negative control samples. Preliminary studies confirmed the adequacy of the samples for testing high molecular weight double-stranded linear DNA targets. All 15 PCR-based assays detected 5,000 copies or less of the SV40 genome spiked into 2 mug of WI-38 DNA. A high level of specificity and reproducibility was found among the PCR assays performed in most laboratories. However, none of the selected normal human lung tissue or the 25 mesothelioma tumor specimens obtained from archival samples at a single center was reproducibly positive for the presence of SV40 DNA. Further studies are needed to reconcile these results with previous reports of detection of SV40 DNA in tumor specimens.

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