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Correlation of human CD56(+) cell cytotoxicity and IFN-gamma production

  1. Author:
    Derby, E. G.
    Reddy, V.
    Nelson, E. L.
    Kopp, W. C.
    Baseler, M. W.
    Dawson, J. R.
    Malyguine, A. M.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, POB B, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Frederick, MD 21702 USA. Duke Univ, Med Ctr, Durham, NC USA. Malyguine AM NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, POB B, Frederick, MD 21702 USA.
    1. Year: 2001
  2. Journal: Cytokine
    1. 13
    2. 2
    3. Pages: 85-90
  4. Type of Article: Article
  1. Abstract:

    The use of an IFN-gamma ELISPOT assay to evaluate cellular immune responses has gained increasing popularity, especially as a surrogate measure for cytotoxic T lymphocyte (CTL) responses. We have compared the IFN-gamma ELISPOT assay and the traditional Cr-51 release assay for detection of human natural killer (NK) cell activity. The cell populations used for evaluation of these assays included freshly isolated and IL-2- activated peripheral blood mononuclear cells (PBMC), CD56- positive cells were demonstrated to be the primary source of the IFN-gamma signal when PBMC were evaluated with NK-sensitive targets in the IFN-gamma ELISPOT assay. IFN-gamma ELISPOT and Cr-51 release assays showed excellent correlation suggesting that NK activity can be reliably evaluated with methods other than the traditional Cr-51 release assays. (C) 2001 Academic Press.

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