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Comparative feline genomics: A BAC/PAC contig map of the major histocompatibility complex class II region

  1. Author:
    Beck, T. W.
    Menninger, J.
    Voigt, G.
    Newman, K.
    Nishigaki, Y.
    Nash, W. G.
    Stephens, R. M.
    Wang, Y.
    de Jong, P. J.
    O'Brien, S. J.
    Yuhki, N.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Intramural Res Support Program, POB B, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Intramural Res Support Program, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Adv Biomed Comp Ctr, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Lab Gen Divers, Frederick, MD 21702 USA. H&W Cytogenet Serv Inc, Lovettsville, VA 22080 USA. Childrens Hosp Oakland, Res Inst, Oakland, CA 94609 USA. Beck TW NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Intramural Res Support Program, POB B, Frederick, MD 21702 USA.
    1. Year: 2001
  2. Journal: Genomics
    1. 71
    2. 3
    3. Pages: 282-295
  4. Type of Article: Article
  1. Abstract:

    The genome organization of the human major histocompatibility complex (MHC) will be best understood in a comparative evolutionary context. We describe here the construction of a physical map for the feline MHC. A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromosome (PAC) with a genomic representation of 2.5 x and an average insert size of 80 kb. A sequence-ready 660-kb bacterial artificial chromosome/PAC contig map of the domestic cat MHC class II region was constructed with a gene order similar to, but distinct from, that of human and mice: DPB/DPA, Ring3, DMB, TAP1, DOB, DRB2, DRA3, DRB1, DRA2, and DRA1. Fluorescence in situ hybridization analyses of selected class II PAC clones confirmed that the class II region lies in the pericentromeric region of cat chromosome B2. However, apparently unlike the human and mouse MHCs, the domestic cat DRA and DRB genes have undergone multiple duplications and the DQ region has been deleted. (C) 2001 Academic Press.

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