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Capillary Endothelial Cell Tropism of Pvc-211 Murine Leukemia Virus and Its Application For Gene Transduction

  1. Author:
    Masuda, M.
    Hanson, C. A.
    Dugger, N. V.
    Robbins, D. S.
    Wilt, S. G.
    Ruscetti, S. K.
    Hoffman, P. M.

  2. Author Address

    Hoffman PM DEPT VET AFFAIRS MED CTR RES SERV 10 N GREENE ST BALTIMORE, MD 21201 USA DEPT VET AFFAIRS MED CTR RES SERV BALTIMORE, MD 21201 USA NCI MOL ONCOL LAB FREDERICK, MD 21702 USA SAIC LAB CELLULAR BIOCH FREDERICK, MD 21702 USA UNIV MARYLAND SCH MED DEPT PATHOL BALTIMORE, MD 21201 USA UNIV MARYLAND SCH MED DEPT NEUROL BALTIMORE, MD 21201 USA
    1. Year: 1997
  2. Journal: Journal of Virology
    1. 71
    2. 8
    3. Pages: 6168-6173
  4. Type of Article: Article
  1. Abstract:

    PVC-211 murine leukemia virus (MuLV) causes neurodegenerative disease following inoculation of neonatal, but not adult, mice and rats. It was previously shown that tropism from capillary endothelial cells (CEC) was a determinant of the viral neuropathogenicity. In this study, we demonstrate that host age-dependent replication of PVC-211 MuLV in vivo occurs in CEC in the brain as well as in other organs, such as the liver, kidney, and heart. In contrast, primary explant cultures of CEC derived from brains and livers of adult and neonatal rats could be infected by PVC-211 MuLV, suggesting that the age-dependent susceptibility was abrogated in vitro. Although CEC were generally less susceptible to MuLV-mediated age transduction than fibroblasts, treatment of CEC with 2-deoxyglucose followed by inoculation of a PVC-211 MuLV-pseudotyped vector in the absence of heparin improved the transduction efficiency. These observations support the possibility that PVC-211 MuLv may be useful for establishing models of CEC gene transduction. [References: 31]

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