Skip NavigationSkip to Content
Return Return to Search Results

Alternative splicing of the proadrenomedullin gene results in differential expression of gene products

  1. Author:
    Martinez, A.
    Hodge, D. L.
    Garayoa, M.
    Young, H. A.
    Cuttitta, F.

  2. Author Address

    NCI, Dept Cell & Canc Biol, Ctr Canc Res, NIH, Bldg 10, Room 13N262, Bethesda, MD 20892 USA. NCI, Dept Cell & Canc Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NCI, Frederick Canc Res & Dev Ctr, Ctr Canc Res, Expt Immunol Lab, Frederick, MD 21702 USA. Martinez A NCI, Dept Cell & Canc Biol, Ctr Canc Res, NIH, Bldg 10, Room 13N262, Bethesda, MD 20892 USA.
    1. Year: 2001
  2. Journal: Journal of Molecular Endocrinology
    1. 27
    2. 1
    3. Pages: 31-41
  4. Type of Article: Article
  1. Abstract:

    The adrenomedullin (AM) gene codifies for two bioactive peptides, AIM and proAM N-terminal 20 peptide (PAMP). We have found two forms of the AXI mRNA. Form A is devoid of introns and results in a prohormone containing both peptides. Form B retains the third intron, which introduces a premature stop codon, producing a shorter prohormone with only PAMP. Tissues with a higher B/A ratio were more immunoreactive for PAMP than for ANI. The form B message was found in the cytoplasmic compartment, thus excluding that the longer message was a result of contaminating nuclear mRNA. Form B was found in cells that express PAMP but not AM. mRNA expression in a variety of cell lines was investigated by ribonuclease protection assay and form B was found in significant amounts in two of them. Treatments that modify AM expression, such as exposure to hypoxia, were shown to change the B/A ratio and the relative secretion of AM and PAMP, indicating that the splicing mechanism for AM can be modulated and is physiologically relevant. Analysis of the sequence of the third intron and the fourth exon of the AM gene found motifs compatible with a highly regulated alternative splicing mechanism.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel