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Ink-jet printer heads for ultra-small-drop protein crystallography

  1. Author:
    Howard, E. I.
    Cachau, R. E.

  2. Author Address

    NCI, SAIC, Adv Biomed Comp Ctr, 430 Miller Dr, Frederick, MD 21702 USA NCI, SAIC, Adv Biomed Comp Ctr, Frederick, MD 21702 USA IFLYSIB, RA-1900 La Plata, Argentina Inst Genet & Biol Mol & Cellulaire, Illkirch Graffenstaden, France Howard EI NCI, SAIC, Adv Biomed Comp Ctr, 430 Miller Dr, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Biotechniques
    1. 33
    2. 6
    3. Pages: 1302-+
  4. Type of Article: Article
  1. Abstract:

    Mass-produced automated piezoelectric driven picoliter delivery systems (printer heads) are fast, inexpensive, and reliable devices that are capable of delivering a very large range of volumes and are ideally suited for high-throughput protein crystallography studies. We used this technology to set up under-oil crystallization experiments with drop sizes from the 200-nL to 3-muL volume range, commonly used in protein crystallography, and show its application in setting ultra- small (2 nL) drops, the smallest drop volume reported to date for this type of assay.

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