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Reversal by dithiothreitol treatment of the block in murine leukemia virus maturation induced by disulfide cross-linking

  1. Author:
    Campbell, S.
    Oshima, M.
    Mirro, J.
    Nagashima, K.
    Rein, A.

  2. Author Address

    NCI, HIV Drug Resistance Program, SAIC, POB B, Frederick, MD 21702 USA NCI, HIV Drug Resistance Program, SAIC, Frederick, MD 21702 USA NCI, Image Anal Lab, Frederick, MD 21702 USA Rein A NCI, HIV Drug Resistance Program, SAIC, POB B, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Journal of Virology
    1. 76
    2. 19
    3. Pages: 10050-10055
  4. Type of Article: Article
  1. Abstract:

    We previously reported that if murine leukemia virus particles are produced in the presence of the mild oxidizing agent disulfide-substituted benzamide-2, they fail to undergo the normal process of virus maturation. We now show that treatment of these immature particles with a reducing agent (dithiothreitol) induces their maturation in vitro, as evidenced by proteolytic cleavage of Gag, Gag-Pol, and Env proteins and by their morphology. The identification of partial cleavage products in these particles suggests the sequence with which the cleavages occur under these conditions. This may be a useful experimental system for further analysis of retroviral maturation under controlled conditions in vitro.

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