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Retrovirus-specific packaging of aminoacyl-tRNA synthetases with cognate primer tRNAs

  1. Author:
    Cen, S.
    Javanbakht, H.
    Kim, S.
    Shiba, K.
    Craven, R.
    Rein, A.
    Ewalt, K.
    Schimmel, P.
    Musier-Forsyth, K.
    Kleiman, L.

  2. Author Address

    McGill Univ, Jewish Gen Hosp, Lady Davis Inst Med Res, 3755 Cote Ste Catherine Rd, Montreal, PQ H3T 1E2, Canada McGill Univ, Jewish Gen Hosp, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada McGill Univ, Jewish Gen Hosp, McGill AIDS Ctr, Montreal, PQ H3T 1E2, Canada McGill Univ, Dept Med, Montreal, PQ H3T 1E2, Canada McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3T 1E2, Canada Sung Kyun Kwan Univ, Natl Creat Res Initiat Ctr ARS Network, Suwon 440746, Kyunggido, South Korea Japanese Fdn Canc Res, Dept Cell Biol, Toshima Ku, Tokyo 170, Japan Penn State Univ, Milton S Hershey Med Ctr, Dept Biochem & Mol Biol, Hershey, PA 17033 USA NCI, HIVDRP, NIH, Frederick, MD 21702 USA Scripps Clin & Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA Kleiman L McGill Univ, Jewish Gen Hosp, Lady Davis Inst Med Res, 3755 Cote Ste Catherine Rd, Montreal, PQ H3T 1E2, Canada
    1. Year: 2002
  2. Journal: Journal of Virology
    1. 76
    2. 24
    3. Pages: 13111-13115
  4. Type of Article: Article
  1. Abstract:

    The tRNAs used to prime reverse transcription in human immunodeficiency virus type 1 (HIV-1), Rous sarcoma virus (RSV), and Moloney murine leukemia virus (Mo-MuLV) are tRNA(3)(Lys), tRNA(TrP), and tRNA(Pro), respectively. Using antibodies to the three cognate human aminoacyl-tRNA synthetases, we found that only lysyl-tRNA synthetase (LysRS) is present in HIV-1, only tryptophanyl-tRNA synthetase (TrpRS) is present in RSV, and neither these two synthetases nor prolyl-tRNA synthetase (ProRS) is present in Mo-MuLV. LysRS and TrpRS are present in HIV-1 and RSV at approximately 25 and 12 molecules/virion, respectively. These results support the hypothesis that, in HIV-1 and RSV, the cognate aminoacyl-tRNA synthetase may be used as the signal for targeting the selective packaging of primer tRNAs into retroviruses. The absence of ProRS in Mo-MuLV is consistent with reports that selective packaging of tRNA(Pro) in this virus is less important for achieving optimum annealing of the primer to Mo- MuLV genomic RNA.

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