Skip NavigationSkip to Content
Return Return to Search Results

Plant annexins form calcium-independent oligomers in solution

  1. Author:
    Hofmann, A.
    Ruvinov, S.
    Hess, S.
    Schantz, R.
    Delmer, D. P.
    Wlodawer, A.

  2. Author Address

    NCI, Prot Struct Sect, Macromol Crystallog Lab, Frederick, MD 21702 USA NCI, Prot Struct Sect, Macromol Crystallog Lab, Frederick, MD 21702 USA NCI, Biochem Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA NIDDK, Struct Mass Spectrometry Facil, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA CNRS, Inst Biol Mol Plantes, F-67084 Strasbourg, France Univ Calif Davis, Plant Biol Sect, Davis, CA 95616 USA Hofmann A NCI, Prot Struct Sect, Macromol Crystallog Lab, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Protein Science
    1. 11
    2. 8
    3. Pages: 2033-2040
  4. Type of Article: Article
  1. Abstract:

    The oligomeric state in solution of four plant annexins, namely Anx23(Ca3g), Anx24(Ca32), Anx(Gh1), and Anx(Gh2), was characterized by sedimentation equilibrium analysis and gel filtration. All proteins were expressed and purified as amino- terminal His,, fusions. Sequencing of the Anx(Gh1) construct revealed distinct differences with the published sequence. Sedimentation equilibrium analysis of Anx23(Ca38), Anx24(Ca32), and Anx(Gh1) suggests monomer-trimer equilibria for each protein with association constants in the range of 0.9 x 10(10)-1.7 x 10(11) M-2. All four proteins were subjected to analytical gel filtration under different buffer conditions. Observations from this experiment series agree quantitatively with the ultracentrifugation results, and strongly suggest calcium independence of the annexin oligomerization behavior; moreover, binding of calcium ions to the proteins seems to require disassembly of the oligomers. Anx(Gh2) showed a different elution profile than the other plant annexins; while having only a very small trimer content, this annexin seems to exist in a monomer-dimer equilibrium in solution.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel