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Differential display analysis of gene expression in yeast

  1. Author:
    Ivanova, A. V.
    Ivanov, S. V.
  2. Author Address

    NCI, Canc Res Ctr, Immunobiol Lab, Bldg 560,Room 12-71,POB B, Frederick, MD 21702 USA NCI, Canc Res Ctr, Immunobiol Lab, Frederick, MD 21702 USA NCI, Canc Res Ctr, IRSP, SAIC Frederick, Frederick, MD 21702 USA Ivanova AV NCI, Canc Res Ctr, Immunobiol Lab, Bldg 560,Room 12-71,POB B, Frederick, MD 21702 USA
    1. Year: 2002
  1. Journal: Cellular and Molecular Life Sciences
    1. 59
    2. 8
    3. Pages: 1241-1245
  2. Type of Article: Review
  1. Abstract:

    RNA differential display (DD) is a powerful and straightforward method that employs random reverse-transcription polymerase chain reaction amplification of mRNA species with electrophoresis for comparative analysis of two or more transcriptomes. The small yeast genome represents a convenient model for studying basic functions of the eukaryotic genome and simultaneously provides valuable information towards further refinement of this technique. Several examples discussed below illustrate how DD coupled with classical yeast genetic approaches may be used for studying transcriptionally regulated genetic systems.

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