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Real-time gap ligase chain reaction: A rapid semiquantitative assay for detecting p53 mutation at low levels in surgical margins and lymph nodes from resected lung and head and neck tumors

  1. Author:
    Harden, S. V.
    Thomas, D. C.
    Benoit, N.
    Minhas, K.
    Westra, W. H.
    Califano, J. A.
    Koch, W.
    Sidransky, D.

  2. Author Address

    Sidransky, D, Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, 818 Ross Res Bldg,720 Rutland Ave, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA. NCI, HIV Drug Resistance Program, Frederick, MD 21701 USA.
    1. Year: 2004
  2. Journal: Clinical Cancer Research
    1. 10
    2. 7
    3. Pages: 2379-2385
  4. Type of Article: Article
  1. Abstract:

    Purpose: We have developed a real-time semiquantitative gap ligase chain reaction for detecting p53 point mutations at low level in a background of excess of wild-type DNA.Experimental Design: This method was validated by direct comparison to a previously validated but cumbersome phage plaque hybridization assay. Forty-one surgical margins and lymph nodes from 10 cases of head and neck squamous cell carcinoma and lung carcinoma were tested for p53 mutant clones.Results: Both methods detected p53 mutants in margins from 8 of the 10 cases, whereas standard pathology detected cancer cells in only 3 cases. Positive margins included tissue samples with a tumor/normal DNA ratio of up to 1:1000.Conclusions: This novel molecular approach can be performed in <5 h facilitating intraoperative use for realtime surgical resection

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External Sources

  1. View record in Web of Science®
  2. WOS: 000220701700023

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