Skip NavigationSkip to Content
Return Return to Search Results

Polymerase Chain Reaction Single-Strand Conformation Polymorphism Analysis For the Vhl Gene in Chemically Induced Kidney Tumors of Rats Using Intron-Derived Primers

  1. Author:
    Shiao, Y. H.
    Diwan, B. A.
    Perantoni, A. O.
    Calvert, R. J.
    Zbar, B.
    Lerman, M. I.
    Rice, J. M.

  2. Author Address

    Shiao YH NCI FREDERICK CANC RES & DEV CTR COMPARAT CARCINOGENESIS LAB BLDG 538 ROOM 205 FREDERICK, MD 21702 USA SCI APPLICAT INT CORP INTRAMURAL RES SUPPORT PROGRAM FREDERICK, MD USA US FDA CTR FOOD SAFETY & APPL NUTR OFF SPECIAL NUTR LAUREL, MD USA NCI FREDERICK CANC RES & DEV CTR IMMUNOBIOL LAB FREDERICK, MD 21701 USA INT AGCY RES CANC UNIT CARCINOGEN IDENTIFICAT & EVALUAT F-69372 LYON FRANCE
    1. Year: 1997
  2. Journal: Molecular Carcinogenesis
    1. 19
    2. 4
    3. Pages: 230-235
  4. Type of Article: Article
  1. Abstract:

    von Hippel-Lindau (VHL) gene mutations occur throughout three exons including the exon-intron boundaries in human VHL disease-associated and sporadic renal cell carcinomas. To explore the possible role of the VHL gene in chemically induced rat kidney tumors originating from various cell types, more than 150 bp of Fischer 344 and Noble rat VHL intron sequences flanking the three exons was determined by dideoxy sequencing. Five primer sets were selected for polymerase chain reaction amplification of the coding regions of rat VHL exons 1-3 and the exon-intron boundaries. Tissues from 10 renal eosinophilic epithelial tumors induced by N-nitrosoethyl(2-hydroxyethyl)amine, 10 nephroblastomas induced by N-nitroso-N-ethylurea, and seven renal mesenchymal tumors induced by N-nitrosomethyl(methoxymethyl)amine were examined for VHL mutations by polymerase chain reaction-single-strand conformation polymorphism analysis. No mutation was detected in any tumor type, indicating that VHL mutations are not involved in the pathogenesis of rat kidney tumors arising from the distal region of the renal tubules, the metanephric blastema, or stromal tissues of the cortex. (C) 1997 Wiley-Liss. Inc. [References: 31]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel