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HPV-16 L1 VLP vaccine elicits a broad-spectrum of cytokine responses in whole blood

  1. Author:
    Pinto, L. A.
    Castle, P. E.
    Roden, R. B.
    Harro, C. D.
    Lowy, D. R.
    Schiller, J. T.
    Wallace, D.
    Williams, M.
    Kopp, W.
    Frazer, I. H.
    Berzofsky, J. A.
    Hildesheim, A.

  2. Author Address

    SAIC Frederick Inc, NCI Frederick, Frederick, MD 21702 USA. NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Baltimore, MD USA. Univ Queensland, Ctr Immunol & Canc Res, St Lucia, Qld 4067, Australia Pinto, LA, SAIC Frederick Inc, NCI Frederick, Room 120,Bldg 469, Frederick, MD 21702 USA
    1. Year: 2005
    2. Date: MAY 20
  2. Journal: Vaccine
    1. 23
    2. 27
    3. Pages: 3555-3564
  4. Type of Article: Article
  1. Abstract:

    Here, we evaluated innate and adaptive immune system cytokine responses induced by HPV-16 L1 VLP in whole blood (WB) cultures from individuals receiving the vaccine (n = 20) or placebo (n = 4) before and after vaccination. 11 cytokines were measured: IL- 1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, 1L- 10, IL- 12, IFN-gamma, TNF-alpha, and GM-CSF using multiplex bead arrays. Cytokine profiles from WB samples clearly discriminated between vaccine and placebo recipients and between pre and post-vaccination responses. Significant increases in Th1, Th2 and inflammatory cytokines were observed in WB assays following vaccination. Results from WB assays were compared against parallel PBMC-based assays in a subset of patients. Differences between whole blood assay and PBMC were observed, with the highest levels of induction found for WB for several cytokines. Our results indicate that multiplex assays for cytokine profiling in WB are an efficient toot for assessing broad spectrum, innate and adaptive immune responses to vaccines and identifying immunologic correlates of protection in efficacy studies. (c) 2005 Elsevier Ltd. All rights reserved

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External Sources

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  2. DOI: 10.1016/j.vaccine.2005.01.146
  3. View record in Web of Science®
  4. WOS: 000229268700008

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