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Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

  1. Author:
    Zhang, X. M.
    Chen, B. Y.
    Ng, A. H. L.
    Tanner, J. A.
    Tay, D.
    So, K. F.
    Rachel, R. A.
    Copeland, N. G.
    Jenkins, N. A.
    Huang, J. D.

  2. Author Address

    Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China. Univ Hong Kong, Dept Anat, Hong Kong, Hong Kong, Peoples R China. NCI, Mouse Canc Genet Program, Ctr Canc Res, Frederick, MD 21701 USA Huang, JD, Univ Hong Kong, Dept Biochem, Fac Med Bldg,21 Sassoon Rd, Hong Kong, Hong Kong, Peoples R China
    1. Year: 2005
    2. Date: OCT
  2. Journal: Investigative Ophthalmology & Visual Science
    1. 46
    2. 10
    3. Pages: 3515-3520
  4. Type of Article: Article
  1. Abstract:

    PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants.METHODS. The IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity.RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKC alpha antibody ( specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina.CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation

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External Sources

  1. View record in Web of Science®
  2. WOS: 000232112900009

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