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Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system

  1. Author:
    Keng, V. W.
    Yae, K.
    Hayakawa, T.
    Mizuno, S.
    Uno, Y.
    Yusa, K.
    Kokubu, C.
    Kinoshita, T.
    Akagi, K.
    Jenkins, N. A.
    Copeland, N. G.
    Horie, K.
    Takeda, J.

  2. Author Address

    Osaka Univ, Ctr Adv Sci & Innovat, Suita, Osaka 5650871, Japan. Osaka Univ, Dept Social & Environm Med, Grad Sch Med, Suita, Osaka 5650871, Japan. Osaka Univ, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan. NCI, Mouse Canc Genet Program, Frederick, MD 21701 USA Takeda, J, Osaka Univ, Ctr Adv Sci & Innovat, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan
    1. Year: 2005
    2. Date: OCT
  2. Journal: Nature Methods
    1. 2
    2. 10
    3. Pages: 763-769
  4. Type of Article: Article
  1. Abstract:

    Recent consolidation of the whole-genome sequence with genome-wide transcriptome profiting revealed the existence of functional units within the genome in specific chromosomal regions, as seen in the coordinated expression of gene clusters and colocatization of functionally related genes. An efficient region-specific mutagenesis screen would greatly facilitate research in addressing the importance of these clusters. Here we use the 'local hopping' phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. A transgenic mouse containing both transposon (acts as a mutagen) and transposase (recognizes and mobilizes the transposon) was bred for germ-cell transposition events, allowing us to generate many mutant mice. ALL genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region

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External Sources

  1. View record in Web of Science®
  2. WOS: 000232998600016

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