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Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: Partial characterization and separation from interleukin 1 (IL 1)

  1. Author:
    Yoshimura, T.
    Matsushima, K.
    Oppenheim, J. J.
    Leonard, E. J.

  2. Author Address

    NCI, Immunopathol Sect, Immunobiol Lab, Frederick, MD 21701 USA. NCI, Immunoregulat Lab, Biol Response Modifiers Program, Frederick, MD 21701 USA Yoshimura, T, NCI, Immunopathol Sect, Immunobiol Lab, Frederick, MD 21701 USA
    1. Year: 2005
    2. Date: NOV 1
  2. Journal: Journal of Immunology
    1. 175
    2. 9
    3. Pages: 5569-5574
  4. Type of Article: Article
  1. Abstract:

    LPS stimulated human blood mononuclear leukocytes to produce a chemotactic factor for human neutrophils. The effect of LPS was dose-dependent; 10 mu g/ml was optimal for production of chemotactic factor. Chemotactic activity was detected 3 hr after LPS stimulation, and reached its peak at 12 hr. No activity was detected in culture supernatants of unstimulated cells, provided LPS-free media were selected. Isoelectric point of the factor, determined by chromatofocusing, was approximately 8 to 8.5. Molecular weight was approximately 10 kilodaltons by Sephacryl S-200 gel filtration or by HPLC gel filtration on TSK-2000 and -3000 columns in succession. The gel filtration fractions were also assayed for IL 1 activity. The elution position of IL 1 activity corresponded to a m.w. of 18. There was no chemotactic activity in the IL 1 activity peak. Furthermore, highly purified natural IL 1 alpha and -beta and recombinant IL 1 alpha and -beta did not exhibit chemotactic activity for neutrophils in our assay. Among mononuclear leukocytes, the monocyte was the principal producer of neutrophil chemotactic factor. These results suggest that a chemotactic factor for neutrophils, different from IL 1. is produced by LPS-stimulated blood monocytes

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  1. View record in Web of Science®
  2. WOS: 000232786300002

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