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Diagnosis of the small round blue cell tumors using multiplex polymerase chain reaction

  1. Author:
    Chen, Q. R.
    Vansant, G.
    Oades, K.
    Pickering, M.
    Wei, J. S.
    Song, Y. K.
    Monforte, J.
    Khan, J.

  2. Author Address

    NCI, Oncogenom Sect, Pediat Oncol Branch, Ctr Adv Technol, Gaithersburg, MD 20877 USA. NCI, SAIC Frederick Inc, Adv Biomed Comp Ctr, Frederick, MD 21701 USA. Althea Technol, San Diego, CA USA.;Khan, J, NCI, Oncogenom Sect, Pediat Oncol Branch, Ctr Adv Technol, 8717 Grovemont Circle, Gaithersburg, MD 20877 USA.;jmonforte@altheatech.com khanjav@mail.nih.gov
    1. Year: 2007
    2. Date: Feb
  2. Journal: Journal of Molecular Diagnostics
    1. 9
    2. 1
    3. Pages: 80-88
  4. Type of Article: Article
  5. ISSN: 1525-1578
  1. Abstract:

    The small round blue cell tumors of childhood, which include neuroblastoma, rhabdomyosarcoma, non Hodgkin's lymphoma, and the Ewing's family of tumors, are so called because of their similar appearance on routine histology. Using cDNA microarray gene expression profiles and artificial neural networks (ANNs), we previously identified 93 genes capable of diagnosing these cancers. Using a subset of these, together with some additional genes (total 39), we developed a multiplex polymerase chain reaction (PCR) assay to diagnose these cancer types. Blinded testing of 96 new samples (26 Ewing's family of tumors, 29 rhabdomyosarcomas, 24 neuroblastomas, and 17 lymphomas) using ANNs in a complete leave-one-out analysis demonstrated that all except one sample were accurately diagnosed as their respective category. Moreover, using an ANN-based gene minimization strategy in a separate analysis, we found that the top 31 genes could correctly diagnose an 96 tumors. Our results suggest that this molecular test based on a multiplex PCR reaction may assist the physician in the rapid confirmation of the diagnosis of these cancers.

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External Sources

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  2. DOI: 10.2353/jmoldx.2007.060111
  3. View record in Web of Science®
  4. WOS: 000243898200011

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