Skip NavigationSkip to Content
Return Return to Search Results

Multicopy plasmid modification with phage lambda red recombineering

  1. Author:
    Thomason, L. C.
    Costantino, N.
    Shaw, D. V.
    Court, D. L.

  2. Author Address

    NCI, Gene Regulat & Chromosome Biol Lab, Frederick, MD 21702 USA. Colgate Univ, Hamilton, NY 13346 USA.;Thomason, LC, NCI, Gene Regulat & Chromosome Biol Lab, Bldg 539,Rm 243, Frederick, MD 21702 USA.;lthomason@ncifcrf.gov
    1. Year: 2007
    2. Date: Sep
  2. Journal: Plasmid
    1. 58
    2. 2
    3. Pages: 148-158
  4. Type of Article: Article
  5. ISSN: 0147-619X
  1. Abstract:

    Recombineering, in vivo genetic engineering using the bacteriophage lambda Red generalized recombination system, was used to create various modifications of a multicopy plasmid derived from pBR322. All genetic modifications possible on the Escherichia coli chromosome and on bacterial artificial chromosomes (BACs) are also possible on multicopy plasmids and are obtained with similar frequencies to their chromosomal counterparts, including creation of point mutations (5-10% unselected frequency), deletions and substitutions. Parental and recombinant plasmids are nearly always present as a mixture following recombination, and circular multimeric plasmid molecules are often generated during the recombineering. Published by Elsevier Inc.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.plasmid.2007.03.001
  3. View record in Web of Science®
  4. WOS: 000249357200006

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel