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Comparison of the Abbott 7000 and Bayer 340 systems for measurement of hepatitis C virus load

  1. Author:
    Highbarger, H. C.
    Hu, Z. H.
    Kottilil, S.
    Metcalf, J. A.
    Polis, M. A.
    Vasudevachari, M. B.
    Lane, H. C.
    Dewar, R. L.

  2. Author Address

    NCI, SAIC Frederick Inc, Virus Isolat & Serol Lab, Frederick, MD 21702 USA. NIH, NIAID, Immunoregulat Lab, Bethesda, MD 20892 USA.;Highbarger, HC, NCI, SAIC Frederick Inc, Virus Isolat & Serol Lab, Frederick, MD 21702 USA.;hhighbarge@mail.nih.gov
    1. Year: 2007
    2. Date: Sep
  2. Journal: Journal of Clinical Microbiology
    1. 45
    2. 9
    3. Pages: 2808-2812
  4. Type of Article: Article
  5. ISSN: 0095-1137
  1. Abstract:

    This study compared two commercially available assays for the measurement of hepatitis C virus (HCV) RNA levels, the Bayer HCV RNA (version 3.0) branched DNA assay and the Abbott HCV anallyte-specific reagent real-time PCR assay, to assess their quantitative relationships, ease of performance, and time to completion. The study group consisted of randomly selected patients from the NIAID human immunodeficiency virus (HIV) outpatient clinic who were infected with HIV type 1 and HCV. One hundred eighty-four samples from 66 patients coinfected with HIV and HCV receiving treatments under various protocols were analyzed for the correlation and agreement of the results. The results indicated that the two assays correlate well in the overlapping linear ranges of the assays and show good agreement. From the results obtained, we have derived a mathematical formula to compare the viral load results between the two assays, which is given as log(10) Abbott assay measure = 0.032 + 1.01 log(10) Bayer assay measure. Although it is preferable to use the same quantitation assay throughout the course of a patient's treatment, valid comparisons of the HCV RNA levels may be made between the results obtained by either of these assays in the overlapping linear range (615 to 7,700,000 IU/ml).

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External Sources

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  2. DOI: 10.1128/jcm.00202-07
  3. View record in Web of Science®
  4. WOS: 000249506900009

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