Skip NavigationSkip to Content
Return Return to Search Results

Broad Mesodermal and Endodermal Deletion of Nodal at Postgastrulation Stages Results Solely in Left/Right Axial Defects

  1. Author:
    Kumar, A.
    Lualdi, M.
    Lewandoski, M.
    Kuehn, M. R.

  2. Author Address

    Kumar, Amit, Lualdi, Margaret, Kuehn, Michael R.] NCI, Lab Prot Dynam & Signaling, NCI Frederick, NIH, Frederick, MD 21702 USA. [Lewandoski, Mark] NCI, Canc & Dev Biol Lab, NCI Frederick, NIH, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Developmental Dynamics
    1. 237
    2. 12
    3. Pages: 3591-3601
  4. Type of Article: Article
  1. Abstract:

    Nodal signaling is a critical regulator of multiple aspects of early vertebrate development including asymmetry along the left/right (LR) axis. To study Nodal function occurring specifically in the postgastrulation embryo, we have used Cre/loxP based conditional mutagenesis. A floxed allele of Nodal was generated and shown to have wild-type function. This allele was then used in conjunction with the T-Cre line, which expresses Cre recombinase broadly in the mesodermal and definitive endodermal lineages posterior to the cranial region. T-Cre activity leads to complete deletion of Nodal before its normal transient expression in the early somite stage lateral plate mesoderm, thereby causing severe LR developmental defects. No other abnormalities were found, suggesting that Nodal signaling has no additional essential functions in developmental patterning within the extensive mesodermal and endodermal domains marked by T-Cre activity. Developmental Dynamics 237:3591-3601, 2008. Published 2008 Wiley-Liss, Inc.

    See More

  1. Keywords:

External Sources

  1. PMID: 18773491

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel