Skip NavigationSkip to Content
Return Return to Search Results

Transcriptional regulation of fatty acid Translocase/CD36 expression by CCAAT/Enhancer-binding protein alpha

  1. Author:
    Qiao, L. P.
    Zou, C. H.
    Shao, P.
    Schaack, J.
    Johnson, P. F.
    Shao, J. H.

  2. Author Address

    Qiao, Liping, Zou, Chenhui, Shao, Peng, Shao, Jianhua] Univ Kentucky, Grad Ctr Nutr Sci, Lexington, KY 40536 USA. [Schaack, Jerome] Univ Colorado Denver, Dept Microbiol, Aurora, CO 80045 USA. [Schaack, Jerome] Hlth Sci Ctr, Aurora, CO 80045 USA. [Johnson, Peter F.] NCI Frederick, Natl Inst Hlth, Lab Prot Dynam & Signaling, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Journal of Biological Chemistry
    1. 283
    2. 14
    3. Pages: 8788-8795
  4. Type of Article: Article
  1. Abstract:

    Fatty acid translocase (FAT/CD36) plays an important role in facilitating long chain fatty acid transport. FAT/CD36 gene deletion protects mice from high fat diet-induced obesity. In this study we have investigated the regulatory mechanism of FAT/CD36 expression at the transcription level. FAT/CD36 expression was activated during 3T3-L1 adipocyte differentiation, and FAT/CD36 protein levels were positively correlated with CCAAT/enhancer-binding protein alpha(C/EBP alpha) and peroxisome proliferator-activated receptor gamma. However, a negative correlation was detected between FAT/CD36 and C/EBP ss. Overexpression of C/EBP alpha or C/EBP ss increased FAT/CD36 mRNAand protein levels in several types of cells. Restoration of C/EBP alpha or C/EBP ss expression in C/EBP alpha- or C/EBP ss-deficient mouse embryonic fibroblasts increased FAT/CD36 expression. However, in mouse embryonic fibroblasts C/EBP alpha was a more potent activator of FAT/CD36 expression than was C/EBP alpha. Expression of C/EBP ss robustly increased FAT/CD36 proximal promoter- directed luciferase expression in human embryonic kidney 293 cells. A C/EBP-responsive element was identified in the FAT/CD36 promoter by using 5 ' and specific site mutations. The binding of C/EBP alpha in the FAT/CD36 promoter was detected by chromatin immunoprecipitation in 3T3-L1 adipocytes. These results demonstrated that C/EBP alpha regulates FAT/CD36 gene expression at the transcriptional level.

    See More

  1. Keywords:

External Sources

  1. PMID: 18263877

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel