Skip NavigationSkip to Content
Return Return to Search Results

Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma

  1. Author:
    Vistica, D. T.
    Krosky, P. M.
    Kenney, S.
    Raffeld, M.
    Shoemaker, R. H.

  2. Author Address

    Vistica, David T.] NCI, Dev Therapeut Program, Screening Technol Branch, Ft Detrick, MD 21702 USA. [Krosky, Paula M.] NCI, SAIC Frederick Inc, Appl Dev Directorate, Frederick, MD 21701 USA. [Raffeld, Mark] NIH, Natl Canc Inst, Pathol Lab, Bethesda, MD 20892 USA.
    1. Year: 2008
  2. Journal: Journal of Pediatric Hematology Oncology
    1. 30
    2. 1
    3. Pages: 46-52
  4. Type of Article: Article
  1. Abstract:

    Alveolar soft part sarcoma (ASPS), a rare soft tissue sarcoma, is characterized by a chromosomal translocation der(17)t(X,17)(p11,q25) resulting in the production of 2 fusion proteins encoded by regions of the genes for alveolar soft part locus (ASPL) and the transcription factor E3 (TFE3). In this study, polyclonal antibodies were generated to 25 mer peptides encompassing the junctional regions of ASPL-TFE3 type 1 and ASPL-TFE3 type 2. The specificity of the affinity purified antibodies for the synthetic peptides and recombinant expressed ASPL-TFE3 type 1 and ASPL-TFE3 type 2 proteins was evaluated by enzyme-linked immunosorbent assay and was highly fusion type specific. Immunohistochemical staining of formalin-fixed, paraffin-embedded ASPS tumors with the fusion-specific antibodies resulted in intense nuclear staining and differentiation between tumors that express the type 1 protein and tumors that express the type 2 protein. These antibodies will be useful for the differential diagnosis of type 1 and type 2 ASPS and also in the detection of the fusion proteins in biochemical and cell biologic investigations.

    See More

  1. Keywords:

External Sources

  1. PMID: 18176180

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel