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Synthesis and Hybridization Properties of Modified Oligodeoxynucleotides Carrying Non-Natural Bases

  1. Author:
    Avino, A.
    Perez-Rentero, S.
    Garibotti, A. V.
    Siddiqui, M. A.
    Marquez, V. E.
    Eritja, R.

  2. Author Address

    Avino, Anna, Perez-Rentero, Sonia, Garibotti, Alejandra V.; Eritja, Ramon] CSIC, Inst Biomed Res, CIBER BBN Networking Ctr Bioengn Biomat & Nanomed, IQAC, E-08028 Barcelona, Spain. [Siddiqui, Maqbool A.; Marquez, Victor E.] NCI, Med Chem Lab, Ctr Canc Res, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Chemistry & Biodiversity
    1. 6
    2. 2
    3. Pages: 117-126
  4. Type of Article: Article
  1. Abstract:

    The impact of the presence of nonnatural bases on the properties of oligodeoxynucleotides has been studied. First, oligodeoxynucleotides carrying 2'-deoxyzebularine were prepared, and the stability of duplexes carrying this analogue was determined by DNA melting experiments. Melting temperatures and thermodynamic data indicated the preference of 2'-deoxyzebularine for 2'-deoxyguanosine, which behaves as a 2'-deoxycytidine analogue, forming a less stable base pair due to the absence of the amino group at position 4. Moreover, the duplex-hairpin equilibrium of a self-complementary oligodeoxynucleotide carrying several natural and normatural bases including 2'-deoxyzebularine as a central mispair, was studied. Depending on the base present in the middle of the sequence, it is possible to affect the stability of the bimolecular duplex modulating the duplex-hairpin equilibrium. Magnesium ions were shown to stabilize preferentially the bimolecular duplex form. The results indicate the importance of the modifications and the role of cations in shifting the structural equilibrium.

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External Sources

  1. PMID: 19235163

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