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Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design

  1. Author:
    Boltz, V. F.
    Maldarelli, F.
    Martinson, N.
    Morris, L.
    McIntyre, J. A.
    Gray, G.
    Hopley, M. J.
    Kimura, T.
    Mayers, D. L.
    Robinson, P.
    Mellors, J. W.
    Coffin, J. M.
    Palmer, S. E.

  2. Author Address

    [Palmer, Sarah E.] Karolinska Inst, Dept Virol, Swedish Inst Infect Dis & Control, S-17182 Solna, Sweden. [Boltz, Valerie F.; Maldarelli, Frank; Coffin, John M.; Palmer, Sarah E.] NCI, HIV Drug Resistance Program, NIH, Frederick, MD 21701 USA. [Martinson, Neil; McIntyre, James A.; Gray, Glenda] Univ Witwatersrand, Perinatal HIV Res Unit, Johannesburg, South Africa. [Morris, Lynn] Natl Inst Communicable Dis, Johannesburg, South Africa. [Martinson, Neil] Johns Hopkins Univ, Sch Med, Baltimore, MD USA. [Hopley, Mark J.; Kimura, Toshio] Boehringer Ingelheim GmbH & Co KG, Ingelheim, Germany. [Mayers, Douglas L.; Robinson, Patrick] Boehringer Ingelheim Pharmaceut, Ridgefield, CT USA. [Mayers, Douglas L.] Idenix Pharmaceut, Cambridge, MA USA. [Mellors, John W.] Univ Pittsburgh, Pittsburgh, PA USA. [Coffin, John M.] Tufts Univ, Dept Mol Biol & Microbiol, Boston, MA 02111 USA.;Palmer, SE, Karolinska Inst, Dept Virol, Swedish Inst Infect Dis & Control, Nobels Vag 18, S-17182 Solna, Sweden.;sarah.palmer@smi.se
    1. Year: 2010
    2. Date: Mar
  2. Journal: Journal of Virological Methods
    1. 164
    2. 1-2
    3. Pages: 122-126
  4. Type of Article: Article
  5. ISSN: 0166-0934
  1. Abstract:

    Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance. (C) 2009 Elsevier B.V. All rights reserved.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.jviromet.2009.11.025
  3. View record in Web of Science®
  4. WOS: 000276820500020

Library Notes

  1. Fiscal Year: FY2009-2010
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