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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis

  1. Author:
    Subburaman, P.
    Austin, B. P.
    Shaw, G. X.
    Waugh, D. S.
    Ji, X. H.

  2. Author Address

    [Subburaman, Priadarsini; Austin, Brian P.; Shaw, Gary X.; Waugh, David S.; Ji, Xinhua] NCI, Macromol Crystallog Lab, Frederick, MD 21702 USA.;Ji, XH, NCI, Macromol Crystallog Lab, 1050 Boyles St, Frederick, MD 21702 USA.;jix@mail.nih.gov
    1. Year: 2010
    2. Date: May 1
    3. Epub Date: 5/7/2010
  2. Journal: Acta Crystallographica Section F-Structural Biology and Crystallization Communications
    1. 66
    2. Pt 5
    3. Pages: 554-557
  4. Type of Article: Article
  5. ISSN: 1744-3091
  1. Abstract:

    Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was over-produced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 angstrom resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 125, c = 54 angstrom.

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External Sources

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  2. DOI: 10.1107/s1744309110009711
  3. PMID: 20445258
  4. PMCID: PMC2864691
  5. View record in Web of Science®
  6. WOS: 000277217700018

Library Notes

  1. Fiscal Year: FY2009-2010
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