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Simultaneous Recovery of DNA and RNA from Formalin-Fixed Paraffin-Embedded Tissue and Application in Epidemiologic Studies

  1. Author:
    Huang, W. Y.
    Sheehy, T. M.
    Moore, L. E.
    Hsing, A. W.
    Purdue, M. P.

  2. Author Address

    [Huang, Wen-Yi; Moore, Lee E.; Hsing, Ann W.; Purdue, Mark P.] NCI, Div Canc Epidemiol & Genet, NIH, US Dept HHS, Bethesda, MD 20892 USA. [Sheehy, Timothy M.] Sci Applicat Int Corp Frederick Inc, DNA Extract & Staging Lab, NCI Frederick, Frederick, MD USA.;Huang, WY, NCI, Div Canc Epidemiol & Genet, NIH, US Dept HHS, 6120 Execut Blvd,EPS 8110,MSC 7240, Bethesda, MD 20892 USA.;huangw@mail.nih.gov
    1. Year: 2010
    2. Date: Apr
  2. Journal: Cancer Epidemiology Biomarkers & Prevention
    1. 19
    2. 4
    3. Pages: 973-977
  4. Type of Article: Review
  5. ISSN: 1055-9965
  1. Abstract:

    Analysis of DNA, RNA, and protein extracted from tissue specimens in epidemiologic studies is useful for assessing etiologic heterogeneity, mechanisms of carcinogenesis, and biomarkers for prognosis and prediction of treatment responses. Fresh-frozen tissue samples may provide optimal quality nucleic acids, but pose multiple logistical considerations, including rapid access to tissues before histopathologic examination and specialized equipment for freezing, transport, and storage; in addition, morphology is often compromised. In contrast, formalin-fixed paraffin-embedded (FFPE) tissue samples, including enormous archives of existing specimens, represent a valuable source of retrospective biological material for epidemiologic research, although presenting different limitations compared with frozen samples. Recent efforts have made progress toward enhancing the utility of FFPE specimens for molecular analyses, including DNA studies, and increasingly for RNA and other macromolecules. Here, we report the method that we used to simultaneously recover DNA and RNA from FFPE tissue specimens with appreciable quantity and quality and discuss briefly the application of tumor markers in epidemiologic studies. Cancer Epidemiol Biomarkers Prev; 19(4); 973-7. (C)2010 AACR.

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External Sources

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  2. DOI: 10.1158/1055-9965.epi-10-0091
  3. View record in Web of Science®
  4. WOS: 000278484400014

Library Notes

  1. Fiscal Year: FY2009-2010
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