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Interleukin-27, an Anti-HIV-1 Cytokine, Inhibits Replication of Hepatitis C Virus

  1. Author:
    Frank, A. C.
    Zhang, X. Z.
    Katsounas, A.
    Bharucha, J. P.
    Kottilil, S.
    Imamichi, T.

  2. Author Address

    [Bharucha, Jennifer P.; Imamichi, Tomozumi] NCI Frederick, SAIC Frederick Inc, Lab Human Retrovirol, Clin Serv Program,Appl & Dev Directorate, Frederick, MD 21702 USA. [Frank, Astrid C.; Zhang, Xiaozhen; Katsounas, Antonios; Kottilil, Shyamasundaran] NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA.;Imamichi, T, NCI Frederick, SAIC Frederick Inc, Lab Human Retrovirol, Clin Serv Program,Appl & Dev Directorate, Bldg 550,Room 126,POB B, Frederick, MD 21702 USA.;timamichi@mail.nih.gov
    1. Year: 2010
    2. Date: Jun
  2. Journal: Journal of Interferon and Cytokine Research
    1. 30
    2. 6
    3. Pages: 427-431
  4. Type of Article: Article
  5. ISSN: 1079-9907
  1. Abstract:

    Interleukin (IL)-27 is a member of IL-12 family cytokine. We have previously reported that IL-27 inhibits human immunodefi ciency virus type-1 (HIV-1) replication in CD4(+) T cells and monocyte-derived macrophages, even though IL-12 enhances HIV-1 replication in primary CD4(+) T cells. Further study demonstrates that IL-27 induces antiviral genes including RNA-dependent protein kinase, oligoadenylate synthetase, and myxovirus protein in the same manner as interferon (IFN)-alpha. Neutralization assay using anti-IFN antibodies, real-time RT-PCR, and enzyme-linked immunosorbent assay demonstrated that IL-27 induces the antiviral genes without the induction of IFNs. IFN-alpha has been administered to hepatitis C virus (HCV)-infected patients as well as HCV/HIV-1 co-infected patients. Despite the improved immunotherapy, some patients are still failed to respond to the treatment. Since IL-27 induces IFN-alpha-like responses including the induction of antiviral genes, it was speculated that IL-27 may impact the replication of HCV. In this study, we evaluated the role of IL-27 on HCV replication using Huh7.5, an HCV permissive cell line. IL-27 induces STAT-1 and -3 in the cell line, and dose-dependently inhibited HCV. These data suggest that IL-27 may play a role in the development of a novel immunotherapeutic strategy for HCV and HCV/HIV co-infection.

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External Sources

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  2. DOI: 10.1089/jir.2009.0093
  3. View record in Web of Science®
  4. WOS: 000278667400007

Library Notes

  1. Fiscal Year: FY2009-2010
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