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An overview of enzymatic reagents for the removal of affinity tags

  1. Author:
    Waugh, D. S.

  2. Author Address

    NCI, Prot Engn Sect, Macromol Crystallog Lab, Ctr Canc Res, Frederick, MD 21702 USA.;Waugh, DS (reprint author), NCI, Prot Engn Sect, Macromol Crystallog Lab, Ctr Canc Res, POB B, Frederick, MD 21702 USA;waughd@mail.nih.gov
    1. Year: 2011
    2. Date: Dec
  2. Journal: Protein Expression and Purification
    1. 80
    2. 2
    3. Pages: 283-293
  4. Type of Article: Review
  5. ISSN: 1046-5928
  1. Abstract:

    Although they are often exploited to facilitate the expression and purification of recombinant proteins, every affinity tag, whether large or small, has the potential to interfere with the structure and function of its fusion partner. For this reason, reliable methods for removing affinity tags are needed. Only enzymes have the requisite specificity to be generally useful reagents for this purpose. In this review, the advantages and disadvantages of some commonly used endo- and exoproteases are discussed in light of the latest information. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.pep.2011.08.005
  3. View record in Web of Science®
  4. WOS: 000296225800017

Library Notes

  1. Fiscal Year: FY2011-2012
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