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Development and Applications of Enhanced Green Fluorescent Protein Mutants

  1. Author:
    Stauber, R. H.
    Horie, K.
    Carney, P.
    Hudson, E. A.
    Tarasova, N. I.
    Gaitanaris, G. A.
    Pavlakis, G. N.
    1. Year: 1998
  1. Journal: Biotechniques
    1. 24
    2. 3
    3. Pages: 462 ff.
  2. Type of Article: Article
  1. Abstract:

    The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) Florescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells. [References: 33]

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