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A PLPPV sequence in the p8 region of Gag provides late domain function for mouse mammary tumor virus

  1. Author:
    Coren,Lori
    Nagashima,Kunio
    Ott, David E

  2. Author Address

    AIDS and Cancer Virus Program, National Cancer Institute at Frederick, Frederick, MD, 21702-1201, USA. Electronic address: corenlv@mail.nih.gov., Advanced Technology Program, National Cancer Institute at Frederick, Frederick, MD, 21702-1201, USA.,
    1. Year: 2019
    2. Date: Sep
    3. Epub Date: 2019 07 19
  2. Journal: Virology
    1. 535
    2. Pages: 272-278
  4. Type of Article: Article
  5. ISSN: 0042-6822
  1. Abstract:

    The late (L) domain sequence used by mouse mammary tumor virus (MMTV) remains undefined. Similar to other L domain-containing proteins, MMTV p8 and p14NC proteins are monoubiquitinated, suggesting L domain function. Site-directed mutagenesis of p8, PLPPV, and p14NC, PLPPL, sequences in MMTV Gag revealed a requirement only for the PLPPV sequence in virion release in a position-dependent manner. Electron microscopy of a defective Gag mutant confirmed an L domain budding defect morphology. The equine infectious anemia virus (EIAV) YPDL core L domain sequence and PLPPV provided L domain function in reciprocal MMTV and EIAV Gag exchange mutants, respectively. Alanine scanning of the PLPPV sequence revealed a strict requirement for the valine residue but only minor requirements for any one of the other residues. Thus, PLPPV provides MMTV L domain function, representing a fourth type of retroviral L domain that enables MMTV Gag proteins to co-opt cellular budding pathways for release. Copyright © 2019 Elsevier Inc. All rights reserved.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.virol.2019.07.015
  3. PMID: 31357166
  4. View record in Web of Science®
  5. WOS: 000483007400029
  6. PII : S0042-6822(19)30190-4

Library Notes

  1. Fiscal Year: FY2018-2019
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