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Assessing Antigen-Specific Cellular Immune Responses upon HIV /SIV Plasmid DNA Vaccination in the Nonhuman Primate Model

  1. Author:
    Hu,Xintao
    Felber,Barbara
    Valentin,Antonio

  2. Author Address

    Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD, USA. xintao.hu@nih.gov., Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD, USA.,
    1. Year: 2021
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 2197
    2. Pages: 113-131
  4. Type of Article: Book Chapter
  5. ISSN: 978-1-0716-0871-5
  1. Abstract:

    Reliable detection and quantification of antigen-specific T cells are critical for assessing the immunogenicity of vaccine candidates. In this chapter, we describe the use of ELISpot and flow cytometry-based assays for efficient detection, mapping, and functional characterization of memory T lymphocytes in different tissues of rhesus macaques immunized with plasmid DNA. Flow cytometric assays provide a large amount of information, both phenotypic and functional, about individual cells, while the ELISpot is well suited for high throughput sample screening.

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  1. Keywords:

External Sources

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  2. DOI: 10.1007/978-1-0716-0872-2_6
  3. PMID: 32827134
  4. View record in Web of Science®
  5. WOS: 000687311400007

Library Notes

  1. Fiscal Year: FY2020-2021
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