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Mucosal challenge of Macaca nemestrina with simian immunodeficiency virus (SIV) following SIV nucleocapsid mutant DNA vaccination

  1. Author:
    Gorelick, R. J.
    Lifson, J. D.
    Yovandich, J. L.
    Rossio, J. L.
    Piatak, M.
    Scarzello, A. J.
    Knott, W. B.
    Bess, J. W.
    Fisher, B. A.
    Flynn, B. M.
    Henderson, L. E.
    Arthur, L. O.
    Benveniste, R. E.

  2. Author Address

    NCI, AIDS Vaccine Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NCI, AIDS Vaccine Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NCI, Basic Res Labs, Frederick, MD 21702 USA. NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NCI, Anim Sci Branch, Bethesda, MD 20892 USA.
    1. Year: 2000
  2. Journal: Journal of Medical Primatology
    1. 29
    2. 3-4
    3. Pages: 209-219
  4. Type of Article: Article
  1. Abstract:

    A simian immunodeficiency virus (SIV)(Mne) DNA clone was constructed that produces viruses containing a four amino acid deletion in the second zinc finger of the nucleocapsid (NC) domain of the Gag polyprotein. Viruses produced from this clone, although noninfectious both in vitro and in vivo, complete a majority of the steps in a single retroviral infection cycle. Eight pig-tailed macaques (Macaca nemestrina) were inoculated intramuscularly and subcutaneously three times over the course of 24 weeks with the NC mutant expressing DNA. These macaques, and four controls, were then challenged mucosally (intrarectally) with the homologous virus (SIV Mne CL E11S) and monitored for evidence of infection and clinical disease. Prior to challenge, a measurable humoral immune response was noted in four of eight immunized macaques. After challenge, all 12 macaques became infected, although four immunized animals greatly restricted their viral replication, and one immunized animal that controlled replication remains antibody negative. No disease has been evidence during the 46- week period of monitoring after challenge.

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