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Protocol for cytoskeleton staining of the semi-adherent multiple myeloma cell line RPMI 8226 by immunofluorescence

  1. Author:
    Osei Amponsa,Vasty
    Magidson,Valentin
    Walters,Kylie

  2. Author Address

    Protein Processing Section, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. Electronic address: vasty.oseiamponsa@nih.gov., Optical Microscopy and Image Analysis Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. Electronic address: valentin.magidson@nih.gov., Protein Processing Section, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. Electronic address: kylie.walters@nih.gov.,
    1. Year: 2024
    2. Date: May 02
    3. Epub Date: 2024 05 02
  2. Journal: STAR Protocols
    1. 5
    2. 2
    3. Pages: 103060
  4. Type of Article: Article
  5. Article Number: 103060
  1. Abstract:

    Preservation of fine cellular details of semi-adherent or suspension cells for imaging by immunofluorescence is challenging. This protocol enables staining of floating cells with minimal morphological distortions, as we demonstrate with the semi-adherent multiple myeloma cell line RPMI 8226. We describe steps to better preserve structural details by fixing, permeabilizing, and staining cells in solution, while minimizing the number of centrifugation steps and centrifugation g-force. For complete details on the use and execution of this protocol, please refer to Osei-Amponsa et al.1. Published by Elsevier Inc.

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External Sources

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  2. DOI: 10.1016/j.xpro.2024.103060
  3. PMID: 38700979
  4. PII : S2666-1667(24)00225-9

Library Notes

  1. Fiscal Year: FY2023-2024
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