Skip NavigationSkip to Content
Return Return to Search Results

Interaction of Ly-49D(+) NK cells with H-2D(d) target cells leads to Dap-12 phosphorylation and IFN-gamma secretion

  1. Author:
    Mason, L. H.
    Willette-Brown, J.
    Mason, A. T.
    McVicar, D.
    Ortaldo, J. R.

  2. Author Address

    Mason LH NCI, Expt Immunol Lab, Div Basic Sci, Frederick Canc Res & Dev Ctr Bldg 560,Room 31-93 Frederick, MD 21702 USA NCI, Expt Immunol Lab, Div Basic Sci, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Journal of Immunology
    1. 164
    2. 2
    3. Pages: 603-611
  4. Type of Article: Article
  1. Abstract:

    Murine Ly-49D augments NK cell function upon recognition of target cells expressing H-2D(d). Ly-49D activation is mediated by the immunoreceptor tyrosine-based activation moth-containing signaling moiety Dap-12, In this report we demonstrate that Ly-49D receptor ligation can lead to the rapid and potent secretion of IFN-gamma. Cytokine secretion can be induced from Ly-49D(+) NK cells after receptor ligation,vith Ab or after interaction with target cells expressing their H-2D(d) ligand, Consistent with the dominant inhibitory function of Ly-49G, NK cells coexpressing Ly-49D and Ly-49G show a profound reduction in IFN-gamma secretion after interaction with targets expressing their common ligand, H-2D(d). Importantly, we are able to demonstrate for the first time that effector/target cell interactions using Ly-49D(+) NK cells and H-2Dd targets result in the rapid phosphorylation of Dap-12 However, Dap-12 is not phosphorylated when Ly-49D(+) NK cells coexpress the inhibitory receptor, Ly-49G, These studies are novel in describing Ly-49 activation vs inhibition, where two Ly-49 receptors recognize the same class I ligand, with the dominant inhibitory receptor down-regulating phosphorylation of Dap-12, cytokine secretion, and cytotoxicity in NK cells. [References: 37]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel