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Cell nuclei spin in the absence of lamin B1

  1. Author:
    Ji, J. Y.
    Lee, R. T.
    Vergnes, L.
    Fong, L. G.
    Stewart, C. L.
    Reue, K.
    Young, S. G.
    Zhang, Q. P.
    Shanahan, C. M.
    Lammerding, J.

  2. Author Address

    Partners Res Facil, Cambridge, MA 02139 USA. Brigham & Womens Hosp, Div Cardiovasc, Cambridge, MA 02139 USA. NCI, NIH, Frederick, MD 21702 USA. Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90095 USA. Univ Calif Los Angeles, Dept Human Genet, Los Angeles, CA 90073 USA. Univ Cambridge, Addenbrookes Hosp, Dept Med, Div Cardiovasc Med, Cambridge CB2 2QQ, England.;Lammerding, J, Partners Res Facil, 65 Landsdowne St, Cambridge, MA 02139 USA.;jlammerding@rics.bwh.harvard.edu
    1. Year: 2007
    2. Date: Jul
  2. Journal: Journal of Biological Chemistry
    1. 282
    2. 27
    3. Pages: 20015-20026
  4. Type of Article: Article
  5. ISSN: 0021-9258
  1. Abstract:

    Mutations of the nuclear lamins cause a wide range of human diseases, including Emery-Dreifuss muscular dystrophy and Hutchinson-Gilford progeria syndrome. Defects in A-type lamins reduce nuclear structural integrity and affect transcriptional regulation, but few data exist on the biological role of B-type lamins. To assess the functional importance of lamin B1, we examined nuclear dynamics in fibroblasts from Lmnb1(Delta/Delta) and wild-type littermate embryos by time-lapse videomicroscopy. Here, we report that Lmnb1(Delta/Delta) cells displayed striking nuclear rotation, with similar to 90% of Lmnb1(Delta/Delta) nuclei rotating at least 90 during an 8-h period. The rotation involved the nuclear interior as well as the nuclear envelope. The rotation of nuclei required an intact cytoskeletal network and was eliminated by expressing lamin B1 in cells. Nuclear rotation could also be abolished by expressing larger nesprin isoforms with long spectrin repeats. These findings demonstrate that lamin B1 serves a fundamental role within the nuclear envelope: anchoring the nucleus to the cytoskeleton.

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External Sources

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  2. DOI: 10.1074/jbc.M611094200
  3. View record in Web of Science®
  4. WOS: 000247650600075

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