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Characterization of the Meiosis-Specific Recombinase Dmc1 of Pneumocystis

  1. Author:
    Kutty, G.
    Achaz, G.
    Maldarelli, F.
    Varma, A.
    Shroff, R.
    Becker, S.
    Fantoni, G.
    Kovacs, J. A.

  2. Author Address

    [Achaz, Guillaume] Univ Paris 06, Paris, France. [Maldarelli, Frank] NCI, HIV Drug Resistance Program, NIH, Frederick, MD 21701 USA. [Achaz, Guillaume] Unite Mixte Rech 7138 & Atelier Bioinformat, Paris, France. [Shroff, Robert] NCI, Biochem & Mol Biol Lab, NIH, Bethesda, MD 20892 USA. [Kutty, Geetha; Fantoni, Giovanna; Kovacs, Joseph A.] NIAID, Dept Crit Care Med, NIH, Ctr Clin, Bethesda, MD 20892 USA. [Varma, Ashok] NIAID, Lab Clin Infect Dis, Bethesda, MD 20892 USA. [Becker, Steven] NIAID, Biol Imaging Facil, Bethesda, MD 20892 USA.;Kovacs, JA, Bldg 10,Room 2C145,MSC 1662, Bethesda, MD 20892 USA.;jkovacs@nih.gov
    1. Year: 2010
    2. Date: Dec
  2. Journal: Journal of Infectious Diseases
    1. 202
    2. 12
    3. Pages: 1920-1929
  4. Type of Article: Article
  5. ISSN: 0022-1899
  1. Abstract:

    The life cycle of Pneumocystis, which causes life-threatening pneumonia in immunosuppressed patients, remains poorly defined. In the present study, we have identified and characterized an orthologue of dmc1, a gene specific for meiotic recombination in yeast, in 3 species of Pneumocystis. dmc1 is a single-copy gene that is transcribed as similar to 1.2-kb messenger RNA, which encodes a protein of 336-337 amino acids. Pneumocystis Dmc1 was 61%-70% identical to those from yeast. Confocal microscopy results indicated that the expression of Dmc1 is primarily confined to the cyst form of Pneumocystis. By sequence analysis of 2 single-copy regions of the human Pneumocystis jirovecii genome, we can infer multiple recombination events, which are consistent with meiotic recombination in this primarily haploid organism. Taken together, these studies support the occurrence of a sexual phase in the life cycle of Pneumocystis.

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External Sources

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  2. DOI: 10.1086/657414
  3. View record in Web of Science®
  4. WOS: 000284356300020

Library Notes

  1. Fiscal Year: FY2010-2011
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