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Targeted JAM-C deletion in germ cells by Spo11-controlled Cre recombinase

  1. Author:
    Pellegrini, M.
    Claps, G.
    Orlova, V. V.
    Barrios, F.
    Dolci, S.
    Geremia, R.
    Rossi, P.
    Rossi, G.
    Arnold, B.
    Chavakis, T.
    Feigenbaum, L.
    Sharan, S. K.
    Nussenzweig, A.

  2. Author Address

    [Pellegrini, Manuela; Claps, Giuseppina; Barrios, Florencia; Dolci, Susanna; Geremia, Raffaele; Rossi, Pellegrino; Rossi, Gabriele] Univ Roma Tor Vergata, Dept Publ Hlth & Cellular Biol, I-00133 Rome, Italy. [Orlova, Valeria V.] Leiden Univ, Med Ctr, NL-2300 RC Leiden, Netherlands. [Arnold, Bernd] German Canc Res Ctr, D-69120 Heidelberg, Germany. [Chavakis, Triantafyllos] Univ Dresden, Dept Med, D-01307 Dresden, Germany. [Chavakis, Triantafyllos] Univ Dresden, Inst Physiol, D-01307 Dresden, Germany. [Feigenbaum, Lionel] Sci Applicat Int Corp, Frederick, MD 21702 USA. [Sharan, Shyam K.] NCI, Mouse Canc Genet Program, Ctr Canc Res, Frederick, MD 21702 USA. [Nussenzweig, Andre] NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA.;Pellegrini, M, Univ Roma Tor Vergata, Dept Publ Hlth & Cellular Biol, I-00133 Rome, Italy.;manuelapellegrini@libero.it
    1. Year: 2011
    2. Date: Jan
  2. Journal: Journal of Cell Science
    1. 124
    2. 1
    3. Pages: 91-99
  4. Type of Article: Article
  5. ISSN: 0021-9533
  1. Abstract:

    Meiosis is a crucial process for the production of functional gametes. However, the biological significance of many genes expressed during the meiotic phase remains poorly understood, mainly because of the lethal phenotypes of the knockout mice. Functional analysis of such genes using the conditional knockout approach is hindered by the lack of suitable Cre transgenic lines. We describe here the generation of transgenic mice expressing Cre recombinase under the control of the meiotic Spo11 gene. Using LacZ-R26(loxP) and EYFP-R26(loxP) reporter mice, we show the specific expression and activity of Cre during meiosis in males and females. Spo11(Cre) mice were then crossed with floxed Nbs1 and JAM-C mice to produce conditional knockouts. A strong reduction of Nbs1 and JAM-C protein levels was found in the testis. Although Nbs1-deleted mice developed minor gonadal abnormalities, JAM-C-knockout mice showed a spermiogenetic arrest, as previously described for the null mice. These results provide strong evidence that Spo11(Cre) transgenic mice represent a powerful tool for deleting genes of interest specifically in meiotic and/or in postmeiotic germ cells.

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External Sources

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  2. DOI: 10.1242/jcs.072959
  3. View record in Web of Science®
  4. WOS: 000285242200011

Library Notes

  1. Fiscal Year: FY2010-2011
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