The Illumina BCL Convert v4.0 is a standalone local software app that converts the Binary Base Call (BCL) files produced by Illumina sequencing systems to FASTQ files. BCL Convert v4.0 also provides adapter handling (through masking and trimming), unique molecular identifier (UMI) trimming, and produces metric outputs.
bcl-convert is the successor to bcl-fastq.
Documentation
Slurm script
#!/bin/bash #SBATCH --job-name=bcl-convert #SBATCH --time=1:00:00 #SBATCH --partition=norm #SBATCH --exclusive #SBATCH --ntasks=32 #SBATCH --mem=256GB module load bcl-convert bcl-convert --bcl-input-directory <RunFolder> --output-directory <OutputFolder>
Build instructions for those who are curious
Log in to Illumina support at https://support.illumina.com
Download from https://support.illumina.com/downloads.html
rpm2cpio bcl-convert-4.1.23-2.el8.x86_64.rpm | cpio -idmv
mkdir -p /mnt/nasapps/production/bcl-convert/4.1.23/
mv bcl-convert/bin /mnt/nasapps/production/bcl-convert/4.1.23