The NIH ARAC has developed useful guidelines regarding rodent euthanasia using carbon dioxide
and special guidance for rodent neonates and feti in the intramural research program
The CCR ACUC supports this guidance and has developed the following guidelines that are relevant to NCI’s animal program.
LASP is available to determine the appropriate flow rates for euthanasia chambers and to optimize humane euthanasia practices with laboratory-specific instructions and training. Confirmation of death and proper carcass disposal are essential. Carcasses and body parts are discarded as MPW in accordance with NIH DOHS policies on waste disposal.
General Recommendations for Euthanizing Laboratory Animals
Special Recommendations on Euthanasia of Animals When Chemically Uncontaminated Tissues and/or Immediate Cessation of CNS Metabolism are Required
Special Euthanasia Procedures for mouse fetuses and neonates, based on ARAC Guidelines