The following services are also available through this program:

Generation of large cohorts of GEMs for experimental use

  • In vitro fertilization (IVF), utilizing F1 males to fertilize oocytes from an appropriate wildtype background (e.g. C57BL/6, FVB, DBA, BALB/c and NOD) will generate embryos to be transferred into pathogen free females
  • On average 20 age-matched progeny of the desired phenotype will be produced
  • This procedure is typically completed within 3 months, saving months over traditional breeding approaches
  • Pups will be accompanied by health reports demonstrating their pathogen free status

Microinjection of targeted mouse ES cells into wildtype blastocysts

TMML offers the service for microinjection of already targeted ES cell clones received from the requestor. Upon receipt, a vial is sent for Molecular Testing of Biological Materials (MTBM). Following receipt of clearance, the laboratory will proceed with microinjection of the mESC into blastocysts from the appropriate background. The resulting chimeras will be shipped out to the facility designated by the requestor. Animal health monitoring will be carried out as requested by the recipient animal facility. It is recommended that Karyotypic analysis be performed prior to providing the cells for microinjection since ESC clones with chromosomal aberrations have diminished capability for germline transmission.

DeNovo generation of ES cells from established mouse models

This service offers generation of embryonic stem cells from established mouse models that include one or multiple modified alleles. Upon receipt of the GEM of interest, timed matings are set up by hormonal stimulation and mating of females from the strain. Embryos are collected at 3.5 dpc (blastocyst stage) and cultured until the inter cell mass (ICM) is observed. ES cell clones are identified following disaggregation of the ICM outgrowths, followed by further culture and expansion. Multiple clones are selected for karyotypic analysis. Two male cell lines with normal karyotype will be expanded into stock vials available for blastocyst microinjection or gene targeting.

General Services Information

For any of the following services or information please use the contact information below:

  • Scheduling Service

    To discuss the scheduling of Transgenic and Knock-Out Services.
  • Courier Service

    Courier Service is arranged by Transgenic Mouse Model personnel once a project is scheduled. AHTS has included a statement about weekly courier services. Animals are transported in a temperature-controlled vehicle. Other samples, paperwork, or pick-ups from the NIH-Bethesda campus can be arranged for delivery or return to the NCI-Frederick campus upon request.

Contact Information

Contact Service Contact Information
Roackie Awasthi
Leidos Biomedical Research, Inc. Phone: 301-846-1864
Fax: 301-846-6181
Email: awasthipp@mail.nih.gov
Betty Clift
Administrative and General Information Phone: 301-846-5226
Fax: 301-846-6181
Email: cliftb@mail.nih.gov