Generation of large cohorts of GEMs for experimental use
Microinjection of targeted mouse ES cells into wildtype blastocysts
TMML offers the service for microinjection of already targeted ES cell clones received from the requestor.
Upon receipt, a vial is sent for Molecular Testing of Biological Materials (MTBM). Following receipt of clearance, the laboratory will proceed with microinjection of the mESC into blastocysts from the appropriate background. The resulting chimeras will be shipped out to the facility designated by the requestor. Animal health monitoring will be carried out as requested by the recipient animal facility. It is recommended that Karyotypic analysis be performed prior to providing the cells for microinjection since ESC clones with chromosomal aberrations have diminished capability for germline transmission.
DeNovo generation of ES cells from established mouse models
This service offers generation of embryonic stem cells from established mouse models that include one or multiple modified alleles. Upon receipt of the GEM of interest, timed matings are set up by hormonal stimulation and mating of females from the strain. Embryos are collected at 3.5 dpc (blastocyst stage) and cultured until the inter cell mass (ICM) is observed. ES cell clones are identified following disaggregation of the ICM outgrowths, followed by further culture and expansion. Multiple clones are selected for karyotypic analysis. Two male cell lines with normal karyotype will be expanded into stock vials available for blastocyst microinjection or gene targeting.
For any of the following services or information please use the contact information below: