Frederick National Laboratory for Cancer Research
Building 535, Suite 513
Frederick, MD 21702-1201
Mr. Julian Bess, Jr. received his B.S. in Biology from Virginia Tech in 1977 and his M.S. in Biomedical Sciences from Hood College in 1981.
He began working at the (now) Frederick National Laboratory for Cancer Research in 1977 in the Viral Resources Laboratory and later trained
in the Protein and Immunochemistry Laboratory directed by Dr. Steve Oroszlan. After receiving his M.S., he became the supervisor of the
Biochemistry unit of the Biological Products Laboratory directed by Dr. Larry Arthur. In 1983 he began supervising both the Biochemistry
and Production units which are now collectively known as the Biological Products Core of the AIDS and Cancer Virus Program.
The Biological Products Core provides the AIDS research community with high quality purified preparations of various strains of Human
Immunodeficiency Virus (HIV) and Simian Immunodeficiency Virus (SIV) economically prepared by leveraging the economy of scale. Lot sizes of
up to 30L can be produced and purified. Materials provided include infectious virus or chemically inactivated virus prepared by treatment with
Aldrithiol-2. This chemical inactivation method was developed by AIDS and Cancer Virus Program personnel and eliminates infectivity while
preserving functional surface proteins including envelope glycoproteins, creating a reagent useful for experiments in which non-infectious,
viral particles with authentic envelope glycoprotein structure are required. We also provide non-inactivated preparations of other retroviruses
such as Human T-cell Leukemia Virus (HTLV), Murine Leukemia Virus (MuLV), and Equine Infectious Anemia Virus (EIAV). Since the T-cell lines used
to propagate HIV and SIV also produce microvesicles (non-viral particles that bud from the cell surface) that can contaminate even highly purified
retrovirus preparations, we also produce purified microvesicle preparations from non-infected cell lines for use as a “negative” control reagent
matched to virus preparations of interest.
We also provide virus preparations on small scale (30ml to a few liters) to accommodate the needs of researchers who require smaller amounts of
virus. We prepare these in a variety of formats including: infectious, chemically inactivated (Aldrithiol-2, NEM, and others), or fluorescently
labeled. In cases of mutual interest between the AIDS and Cancer Virus Program and requesting laboratories, we can produce and purify your particular
HIV or SIV with the aim of not only supporting your research project(s), but also eventually making the virus available to the AIDS research community
in general. This can be initiated from your productively infected cell line or, as is often the case, we can derive infected cell lines or cell clones
starting with your infectious stock or infectious molecular clone containing plasmid.
The Biological Products Core also provides reagents used for an in house developed HIV-1 p24 antigen capture immunoassay, originally developed to
monitor virus yields from our production cultures or infectivity assays. These reagents include: the capture antibody (purified mAb against HIV-1 p24),
detect antibody (polyclonal anti-HIV-1 p24), and p24 standard (detergent disrupted HIV-1 with a known p24 concentration), We also supply protocols
describing how we optimize the amount of the key antibody reagents used and perform antigen capture immunoassays. We have several decades of experience
with this assay and can assist you in implementing this assay.
Our main objective is to provide the AIDS research community access to the biological products, most of which are unique, that we efficiently produce.
Most of these can be obtained through a simple Material Transfer Agreement (MTA) between the requesting laboratory and the National Cancer Institute which
we will assist you in requesting. Please contact us if you feel we can be of assistance in your research.