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Gregory Q. Del Prete, Ph.D.

Specimen Support Core

Head, Specimen Support Core
Leidos Biomedical Research, Inc.
Frederick National Laboratory for Cancer Research
Frederick, MD 21702-1201

Tel: 301-846-6306
Fax: 301-846-5588
Email: delpretegq@mail.nih.gov

Biography

Dr. Del Prete received his Ph.D. in Cell and Molecular Biology in 2009 from the University of Pennsylvania, where he studied the molecular mechanisms of coreceptor utilization and cell entry for HIV and SIV. That same year, he joined the AIDS and Cancer Virus Program as a post-doctoral fellow in the Retroviral Pathogenesis Section under Dr. Jeffrey Lifson, where he still serves as a Staff Scientist conducting research focused on the development and utilization of non-human primate models for studies of AIDS virus transmission, pathogenesis, and persistence and for evaluations of retroviral treatment, prevention, and eradication strategies. In 2013, Dr. Del Prete was appointed Head of the Specimen Support Core.

Research Description

In August 1998 an internal ACVP core was created to provide support for processing and assaying specimens derived from vaccine and pathogenesis studies conducted in non-human primates. This core, designated the Specimen Support Core (SSC), is charged with providing the ongoing infrastructure support for non-human primate protocols and now serves as the “clinical laboratory” equivalent for specimens associated with AIDS vaccine and pathogenesis studies in non-human primates conducted by ACVP investigators and collaborators. The SSC receives, accessions, processes, distributes, and stores blood and tissue derived specimens from macaques under study, and maintains detailed databases on these specimens, including current inventories of cryo-preserved samples. The SSC also performs standardized assays including flow cytometry for these samples. Responsibility for preparation of all anti-retroviral drugs for use in ACVP and collaborative non-human primate studies also falls to the SSC. The SSC primarily supports the non-human primate studies of ACVP investigators and their intramural and extramural collaborators.

Recent Publications

  1. Del Prete GQ, Ailers B, Moldt B, Keele BF, Estes JD, Rodriguez A, Sampias M, Oswald K, Fast R, Trubey CM, Chertova E, Smedley J, LaBranche CC, Montefiori DC, Burton DR, Shaw GM, Markowitz M, Piatak M Jr, KewalRamani VN, Bieniasz PD, Lifson JD, Hatziioannou T: Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host Microbe 16(3):412-418, 2014. doi: 10.1016/j.chom.2014.08.003. PMID: 25211081
  2. Del Prete GQ, Shoemaker R, Oswald K, Lara A, Trubey CM, Fast R, Schneider DK, Kiser R, Coalter V, Wiles A, Wiles R, Freemire B, Keele BF, Estes JD, Quiñones OA, Smedley J, Macallister R, Sanchez RI, Wai JS, Tan CM, Alvord WG, Hazuda DJ, Piatak M Jr, Lifson JD: Effect of SAHA administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected Indian rhesus macaques. Antimicrob Agents Chemother. 2014 Sep 2. pii: AAC.03746-14. [Epub ahead of print] PMID: 25182644
  3. Sandler NG, Bosinger SE, Estes JD, Zhu RT, Tharp GK, Boritz E, Levin D, Wijeyesinghe S, Makamdop KN, Del Prete GQ, Hill BJ, Timmer JK, Reiss E, Yarden G, Darko S, Contijoch E, Todd JP, Silvestri G, Nason M, Norgren Jr RB, Keele BF, Rao S, Langer JA, Lifson JD, Schreiber G, Douek DC: Type I interferon responses in rhesus macaques prevent SIV infection and slow disease progression. Nature 511(7511):601-605, 2014. Epub 2014 Jul 9. PMID: 25043006
  4. Do T, Murphy G, Earl LA, Del Prete GQ, Grandinetti G, Estes JD, Rao P, Trubey CM, Thomas J, Li G, Spector J, Bliss D, Nath A, Lifson JD, Subramaniam S: Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. J Virol 88(18):10327-10339, 2014. doi: 10.1128/JVI.00788-14. Epub 2014 Jun 25. PMID: 24965444
  5. Hatziioannou T, Del Prete GQ, Keele BF, Estes JD, McNatt MW, Bitzegeio J, Raymond A, Rodriguez A, Schmidt F, Mac Trubey C, Smedley J, Piatak M Jr, KewalRamani VN, Lifson JD, Bieniasz PD: HIV-1 induced AIDS in monkeys. Science 344(6190):1401-1405, 2014. doi: 10.1126/science.1250761. PMID: 24948736
  6. Del Prete GQ, Park H, Fennessey CM, Reid C, Lipkey L, Lewman L, Oswald K, Kahl C, Piatak M Jr, Quinones OA, Alvord WG, Smedley J, Estes JD, Lifson JD, Picker LJ, Keele BF: Molecularly tagged SIVmac239 synthetic swarm for tracking independent infection events. J Virol 88(14):8077-8090, 2014. doi: 10.1128/JVI.01026-14. Epub 2014 May 7. PMID: 24807714
  7. Smedley J, Turkbey IB, Bernardo ML, Del Prete GQ, Estes JD, Griffiths GL, Kobayashi H, Choyke PL, Lifson JD, Keele BF: Tracking the luminal exposure and lymphatic drainage pathways of intravaginal and intrarectal inocula used in nonhuman primate models of HIV transmission. PLoS One 9(3):e92830, 2014. doi: 10.1371/journal.pone.0092830. eCollection 2014. PMID: 24667371
  8. Del Prete GQ, Lifson JD: Considerations in the development of nonhuman primate models of combination antiretroviral therapy for studies of AIDS virus suppression, residual virus, and curative strategies. Curr Opin HIV AIDS 8(4):262-272, 2013. doi:10.1097/COH.0b013e328361cf40. Epub 2013 May 21. PMID: 23698559
  9. Apps R, Qi Y, Carlson JM, Chen H, Gao X, Thomas R, Yuki Y, Del Prete GQ, Goulder P, Brumme ZL, Brumme CJ, John M, Mallal S, Nelson G, Bosch R, Heckerman D, Stein JL, Soderberg KA, Moody MA, Denny TN, Zeng X, Fang J, Moffett A, Lifson JD, Goedert JJ, Buchbinder S, Kirk GD, Fellay J, McLaren P, Deeks SG, Pereyra F, Walker B, Michael NL, Weintrob A, Wolinsky S, Liao W, Carrington M: Influence of HLA-C Expression level on HIV Control. Science 340(6128):87-91, 2013. doi: 10.1126/science.1232685.
  10. Del Prete GQ, Scarlotta M, Newman L, Reid C, Parodi LM, Roser JD, Oswald K, Marx PA, Miller CJ, Desrosiers RC, Barouch DH, Pal R, Piatak M Jr, Chertova E, Giavedoni LD, O’Connor DH, Lifson JD, Keele BK: Comparative characterization of transfection- and infection –derived SIV challenge stocks for in vivo non-human primate studies. J Virol 87(8):4584-4595, 2013, doi: 10.1128/JVI.03507-12. Epub 2013 Feb 13. PMID: 23408608
  11. Kearney MF, Lee K, Bagni RK, Wiegand A, Spindler J, Maldarelli F, Pinto PA, Linehan WM, Vocke CD, Delviks-Frankenberry KA, Devere White RW, Del Prete GQ, Mellors JW, Lifson JD, Kewalramani VN, Pathak VK, Coffin JM, Le Grice SF: Nucleic acid, antibody, and virus culture methods to detect xenotropic MLV-related virus in human blood samples. Adv Virol 2011:272193, 2011. Epub 2011 Nov 17. PMID: 22312339
  12. Del Prete GQ, Kearney MF, Spindler J, Wiegand A, Chertova E, Roser JD, Estes JD, Hao XP, Trubey CM, Lara A, Lee K, Chaipan C, Bess JW Jr, Nagashima K, Keele BF, Pung R, Smedley J, Pathak VK, Kewalramani VN, Coffin JM, Lifson JD: Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques. J Virol 86(6):3152-3166, 2012. Epub 2012 Jan 11. PMID: 22238316
  13. Taaffe JE, Bosinger SE, Del Prete GQ, Else JG, Ratcliffe S, Ward CD, Migone T, Paiardini M, Silvestri G: CCR5 blockade is well tolerated and induces changes in the tissue distribution of CCR5+ and CD25+ T cells in healthy, SIV-uninfected rhesus macaques. J Med Primatol 41(1):24-42, 2012. doi: 10.1111/j.1600-0684.2011.00521.x. Epub 2011 Nov 14. PMID: 22077380

Staffing

  • Vicky J. Coalter, Research Associate III
  • Donald G. Johnson, M.S., Research Associate III
  • Adam C. Wiles, Research Associate II
  • Rodney W. Wiles, Research Associate I