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Gregory Q. Del Prete, Ph.D.

Specimen Support Core

Head, Specimen Support Core

Frederick National Laboratory for Cancer Research
Frederick, MD 21702-1201

Tel: 301-846-6306
Fax: 301-846-5588
Email: delpretegq@mail.nih.gov

Biography

Dr. Del Prete received his Ph.D. in Cell and Molecular Biology in 2009 from the University of Pennsylvania, where he studied the molecular mechanisms of coreceptor utilization and cell entry for HIV and SIV. That same year, he joined the AIDS and Cancer Virus Program as a post-doctoral fellow in the Retroviral Pathogenesis Section under Dr. Jeffrey Lifson, where he still serves as a Staff Scientist conducting research focused on the development and utilization of non-human primate models for studies of AIDS virus transmission, pathogenesis, and persistence and for evaluations of retroviral treatment, prevention, and eradication strategies. In 2013, Dr. Del Prete was appointed Head of the Specimen Support Core.

Research Description

In August 1998 an internal ACVP core was created to provide support for processing and assaying specimens derived from vaccine and pathogenesis studies conducted in non-human primates. This core, designated the Specimen Support Core (SSC), is charged with providing the ongoing infrastructure support for non-human primate protocols and now serves as the “clinical laboratory” equivalent for specimens associated with AIDS vaccine and pathogenesis studies in non-human primates conducted by ACVP investigators and collaborators. The SSC receives, accessions, processes, distributes, and stores blood and tissue derived specimens from macaques under study, and maintains detailed databases on these specimens, including current inventories of cryo-preserved samples. The SSC also performs standardized assays including flow cytometry for these samples. Responsibility for preparation of all anti-retroviral drugs for use in ACVP and collaborative non-human primate studies also falls to the SSC. The SSC primarily supports the non-human primate studies of ACVP investigators and their intramural and extramural collaborators.

Recent Publications

  1. Hatziioannou T, Del Prete GQ, Keele BF, Estes JD, McNatt MW, Bitzegeio J, Raymond A, Rodriguez A, Schmidt F, Mac Trubey C, Smedley J, Piatak M Jr, KewalRamani VN, Lifson JD, Bieniasz PD: HIV-1-induced AIDS in monkeys. Science. 2014 Jun 20;344(6190):1401-5. doi: 10.1126/science.1250761.
  2. Del Prete GQ, Park H, Fennessey CM, Reid C, Lipkey L, Newman L, Oswald K, Kahl C, Piatak M Jr, Quiñones OA, Alvord WG, Smedley J, Estes JD, Lifson JD, Picker LJ, Keele BF: Molecularly Tagged Simian Immunodeficiency Virus SIVmac239 Synthetic Swarm for Tracking Independent Infection Events, J Virol. 2014 Jul 15;88(14):8077-90. doi: 10.1128/JVI.01026-14. Epub 2014 May 7.PMID: 24807714
  3. Smedley J, Turkbey B, Bernardo ML, Del Prete GQ, Estes JD, Griffiths GL, Kobayashi H, Choyke PL, Lifson JD, Keele BF: Tracking the luminal exposure and lymphatic drainage pathways of intravaginal and intrarectal inocula used in nonhuman primate models of HIV transmission. PLoS One. 2014 Mar 25;9(3):e92830. doi: 10.1371/journal.pone.0092830. eCollection 2014. PMID: 24667371
  4. Del Prete GQ, Lifson JD: Considerations in the development of nonhuman primate models of combination antiretroviral therapy for studies of AIDS virus suppression, residual virus, and curative strategies. Curr Opin HIV AIDS. 2013 Jul;8(4):262-72. doi: 10.1097/COH.0b013e328361cf40. Review.PMID: 23698559
  5. Apps R, Qi Y, Carlson JM, Chen H, Gao X, Thomas R, Yuki Y, Del Prete GQ, Goulder P, Brumme ZL, Brumme CJ, John M, Mallal S, Nelson G, Bosch R, Heckerman D, Stein JL, Soderberg KA, Moody MA, Denny TN, Zeng X, Fang J, Moffett A, Lifson JD, Goedert JJ, Buchbinder S, Kirk GD, Fellay J, McLaren P, Deeks SG, Pereyra F, Walker B, Michael NL, Weintrob A, Wolinsky S, Liao W, Carrington M: Influence of HLA-C expression level on HIV control. Science. 2013 Apr 5;340(6128):87-91.
  6. Do T, Murphy G, Earl LA, Del Prete GQ, Grandinetti G, Li G, Estes JD, Rao P, Trubey CM, Thomas J, Spector J, Bliss D, Nath A, Lifson JD, Subramaniam S: 3D imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. J Virol. 2014 Jun 25. pii: JVI.00788-14. [Epub ahead of print] PMID: 24965444.
  7. Del Prete GQ, Scarlotta M, Newman L, Reid C, Parodi LM, Roser JD, Oswald K, Marx PA, Miller CJ, Desrosiers RC, Barouch DH, Pal R, Piatak M Jr, Chertova E, Giavedoni LD, O'Connor DH, Lifson JD, Keele BF: Comparative characterization of transfection- and infection-derived simian immunodeficiency virus challenge stocks for in vivo nonhuman primate studies. J Virol. 2013 Apr;87(8):4584-95. doi: 10.1128/JVI.03507-12. Epub 2013 Feb 13.
  8. Del Prete GQ, Kearney MF, Spindler J, Wiegand A, Chertova E, Roser JD, Estes JD, Hao XP, Trubey CM, Lara A, Lee K, Chaipan C, Bess JW Jr, Nagashima K, Keele BF, Macallister R, Smedley J, Pathak VK, Kewalramani VN, Coffin JM, Lifson JD: Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques. J Virol. 2012 Mar;86(6):3152-66. doi: 10.1128/JVI.06886-11. Epub 2012 Jan 11. PMID: 22238316
  9. Minang JT, Trivett MT, Barsov EV, Del Prete GQ, Trubey CM, Thomas JA, Gorelick RJ, Piatak M Jr, Ott DE, Ohlen C. TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection. Virology. 2011 Jan 5;409(1):132-40. doi: 10.1016/j.virol.2010.10.005. Epub 2010 Oct 28.

Staffing

  • Vicky J. Coalter, Research Associate III
  • Donald Johnson, Research Associate II
  • Adam C. Wiles, Research Associate I
  • Rodney W. Wiles, Research Associate I