Photo of Mr. Trubey

C. Mac Trubey, M.S.

Associate Scientist and Head, Cellular Immunity Core

Leidos Biomedical Research, Inc.
Frederick National Laboratory for Cancer Research
Building 535, Room 532
Frederick, MD 21702-1201

Tel: 301-846-5320
Fax: 301-846-5588


Mr. Mac Trubey received his B.S. in 1995 from the University of MD and his M.S. degree in 2002 from Hood College. In 1993, he began his research career at the University of MD Cancer Center, under Dr. Nicholas Bachur. In 1996, Mr. Trubey began working at NCI-Frederick, in an HIV research laboratory under Dr. Gene Shearer and moved to the Retroviral Pathogenesis Section of the AIDS and Cancer Virus Program in 2002, under Dr. Jeff Lifson. With over 20 years of advanced flow cytometry and immunology experience, Mr. Trubey has served as the Head of the Cellular Immunity Core, since its establishment in 2009.

Core Descriptions

James Thomas, Mac Trubey, and Abigail Lara
James Thomas, Scientist I; Charles Mac Trubey, Head, CIC; Abigail Lara, Res. Assoc. II

Mission: The mission of the Cellular Immunity Core is to provide ACVP investigators and collaborators with quantitative and multi-parametric cellular analysis and cell separation using advanced flow cytometry methods and instrumentation.

Key Enabling Technologies: Polychromatic flow cytometry, infectious cell sorting, advanced phenotypic and functional cellular immune monitoring, complex multi-parameter flow cytometry data analysis. Advanced instrumentation include: BD FACSAria II 3-laser, 12-color infectious cell sorter equipped with an aerosol management system, sample temperature control module, and a cell deposition unit for single-cell and plate/slide-based sorting; BD Fortessa X-20 (20-color) and LSR-II (16-color) 4-laser, digital flow cytometers equipped with high-sensitivity green lasers and 96-/384-well plate autosamplers; and a BD FACSVerse 3-laser, 8-color flow cytometer equipped with a 96-well plate/40-tube autosampler and a volumetric sensor for performing absolute cell counting.

Scope of Activities: The activities of the CIC include:

i) providing state-of-the-art phenotypic and functional immune monitoring for internal and collaborative non-human primate (NHP) studies

ii) providing cutting-edge flow cytometry support and data analysis to the ACVP and collaborators

iii) determining the needs of investigators and advise as to the utility of flow cytometry towards their specific research endeavors and problems

iv) providing technical expertise and training to ACVP investigators and staff in the application of basic and advanced flow cytometry techniques

v) developing innovations to address the current and future immunology and flow cytometry needs of the ACVP

Workstation of CIC Workstation of CIC showing BD Fortessa X-20
Workstation of CIC BD FACSVerse 3-laser Scientist in a hazordous suit preforming experiment

Recent Publications

  1. Del Prete GQ, Ailers B, Moldt B, Keele BF, Estes JD, Rodriguez A, Sampias M, Oswald K, Fast R, Trubey CM, Chertova E, Smedley J, LaBranche CC, Montefiori DC, Burton DR, Shaw GM, Markowitz M, Piatak M Jr, KewalRamani VN, Bieniasz PD, Lifson JD, Hatziioannou T: Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host Microbe 16(3):412-418, 2014. doi: 10.1016/j.chom.2014.08.003. PMID: 25211081
  2. Del Prete GQ, Shoemaker R, Oswald K, Lara A, Trubey CM, Fast R, Schneider DK, Kiser R, Coalter V, Wiles A, Wiles R, Freemire B, Keele BF, Estes JD, Quiñones OA, Smedley J, Macallister R, Sanchez RI, Wai JS, Tan CM, Alvord WG, Hazuda DJ, Piatak M Jr, Lifson JD: Effect of SAHA administration on the residual virus pool in a model of combination antiretroviral therapy-mediated suppression in SIVmac239-infected Indian rhesus macaques. Antimicrob Agents Chemother. 2014 Sep 2. pii: AAC.03746-14. [Epub ahead of print] PMID: 25182644
  3. Do T, Murphy G, Earl LA, Del Prete GQ, Grandinetti G, Estes JD, Rao P, Trubey CM, Thomas J, Li G, Spector J, Bliss D, Nath A, Lifson JD, Subramaniam S: Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. J Virol 88(18):10327-10339, 2014. doi: 10.1128/JVI.00788-14. Epub 2014 Jun 25. PMID: 24965444
  4. Hatziioannou T, Del Prete GQ, Keele BF, Estes JD, McNatt MW, Bitzegeio J, Raymond A, Rodriguez A, Schmidt F, Mac Trubey C, Smedley J, Piatak M Jr, KewalRamani VN, Lifson JD, Bieniasz PD: HIV-1 induced AIDS in monkeys. Science 344(6190):1401-1405, 2014. doi: 10.1126/science.1250761. PMID: 24948736
  5. Mendoza D, Migueles SA, Rood JE, Peterson B, Johnson S, Doria-Rose N, Schneider D, Rakasz E, Trivett MT, Trubey CM, Coalter V, Hallahan CW, Watkins D, Franchini G, Lifson JD, Connors M: Cytotoxic capacity of SIV-specific CD8(+) T cells against primary autologous targets correlates with immune control in SIV-infected rhesus macaques. PLoS Pathog 9(2):e1003195, 2013. doi: 10.1371/journal.ppat.1003195. Epub 2013 Feb 28. PMID: 23468632
  6. Tabb B, Morcock DR, Trubey CM, Quiñones OA, Hao XP, Smedley J, Macallister R, Piatak M Jr, Harris LD, Paiardini M, Silvestri G, Brenchley JM, Alvord WG, Lifson JD, Estes JD: Reduced inflammation and lymphoid tissue immunopathology in rhesus macaques receiving anti-tumor necrosis factor treatment during primary simian immunodeficiency virus infection. J Infect Dis 207(6):880-892, 2013 doi: 10.1093/infdis/jis643. Epub 2012 Oct 19. PMID: 23087435
  7. Del Prete GQ, Kearney MF, Spindler J, Wiegand A, Chertova E, Roser JD, Estes JD, Hao XP, Trubey CM, Lara A, Lee K, Chaipan C, Bess JW Jr, Nagashima K, Keele BF, Pung R, Smedley J, Pathak VK, Kewalramani VN, Coffin JM, Lifson JD: Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques. J Virol 86(6):3152-3166, 2012. Epub 2012 Jan 11. PMID: 22238316


  • James Thomas, Ph.D., Scientist I
  • Abigail B. Lara, Research Associate II

Reagents/Protocols available for non-commercial use

(please submit requests to Mac Trubey)
  • SIV ENV Monoclonal Antibody (Clone 7D3) labeled with Alexa Fluor 647
  • SIV GAG Monoclonal Antibody labeled with FITC
  • Non-Human Primate Flow Cytometry and Immunology Protocols
    • Polychromatic Intracellular Cytokine Assay
    • Custom Polychromatic Panel Design and Reagent QC/Titration
    • Polychromatic Surface and Intracellular Phenotyping Assays to Monitor:
      • HIV and SIV Infection
      • Cellular Immune Responses
      • Transcription Factors
      • Cell Viability, Apoptosis and Death
      • Enzymes and Enzymatic Activity
      • Protein Phosphorylation
    • Cell Subset and Activation Panels for Monitoring:
      • Activated Memory T cells
      • Activated monocytes, macrophages and granulocytes
      • Detailed NHP B cell populations
      • NHP NK populations
    • Polychromatic Acetylated-Histone Leukocyte Detection Assay
    • Polychromatic Flow Cytometry RNA Detection Assay
    • NHP Absolute Counting Assays to Detect:
      • Memory/Naïve T cells
      • NK cells
      • B cells
      • Macrophages
      • Monocytes
      • Granulocytes