AIDS and Cancer Virus Program

Biography

Mr. Mac Trubey received his B.S. in 1995 from the University of Maryland and his M.S. degree in 2002 from Hood College. In 1993, he began his research career at the University of Maryland Cancer Center, under Dr. Nicholas Bachur. In 1996, Mr. Trubey began working at Frederick National Laboratory for Cancer Research, in an HIV research laboratory under Dr. Gene Shearer and moved to the Retroviral Pathogenesis Section of the AIDS and Cancer Virus Program in 2002, under Dr. Jeff Lifson. With over 14 years of advanced flow cytometry and immunology experience, Mr. Trubey has served as the Head of the Cellular Immunity Core, since its establishment in 2009.

Core Descriptions

The mission of the Cellular Immunity Core is to provide ACVP investigators and collaborators with quantitative and multi-parametric cellular analysis and cell separation using advanced flow cytometry methods and instrumentation.

The CIC is a centralized flow cytometry and cellular immunology resource for the ACVP; providing instrumentation and essential services for NHP studies, investigators and collaborators. The activities of the CIC include: providing state-of-the-art phenotypic and functional immune monitoring for internal and collaborative NHP studies; identifying cost-effective reagents and sourcing or producing custom reagents where needed; providing cutting-edge flow cytometry support and data analysis to the ACVP and collaborators; determining the needs of investigators and providing advice as to the utility of flow cytometry for their specific research problems and goals; providing technical expertise and training to ACVP investigators and staff in the application of basic and advanced flow cytometry techniques; and developing innovations to address the current and future flow cytometry needs of the ACVP.

Advanced instrumentation maintained by the CIC include: a 3-laser, 12-color Becton Dickinson FACSAria II cell sorter equipped with an aerosol management system, sample temperature control module, and a cell deposition unit for single-cell and plate/slide sorting; a 4-laser, 16-color digital Becton Dickinson LSR-II analytical flow cytometer equipped with a green laser and plate autosampler; and a 2-laser, 4-color analog Becton Dickinson FACSCalibur flow cytometer equipped with a 40-tube carousel.

Recent Publications

1. Del Prete GQ, Kearney MF, Spindler J, Wiegand A, Chertova E, Roser JD, Estes JD, Hao XP, Trubey CM, Lara A, Lee K, Chaipan C, Bess JW Jr, Nagashima K, Keele BF, Pung R, Smedley J, Pathak VK, Kewalramani VN, Coffin JM, Lifson JD: Restricted replication of xenotropic murine leukemia virus-related virus in pigtailed macaques. J Virol 86(6):3152-3166, 2012. Epub 2012 Jan 11. PMID: 22238316
2. Minang JT, Trivett MT, Barsov EV, Del Prete GQ, Trubey CM, Thomas JA, Gorelick RJ, Piatak M Jr, Ott DE, Ohlen C: TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection. Virology 409(1):132-40, 2011. Epub 2010 Oct 28. PMID: 21035160.
3. Felts R, Narayan K, Estes J, Shi D, Trubey C, Fu J, Hartnell L, Ruthel G, Schneider D, Nagashima K, Bess J Jr, Bavari S, Lowekamp B, Bliss D, Lifson J, Subramaniam S: 3D visualization of HIV transfer at the virological synapse between dendritic cells and T-cells. PNAS 107:13336-41, 2010.
4. Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M Jr, Roederer M, Lifson JD, Ott D, Ohlen C: Distribution, persistence and efficacy of adoptively transferred central and effector memory-derived autologous SIV-specific CD8+ T cell clones in rhesus macaques during acute infection. J Immunol 184(1):315-326, 2010. Epub 2009 Nov 30. PMID: 19949091.
5. Hatziioannou T, Ambrose Z, Chung NPY, Piatak MJr, Yuan F, Trubey CM, Coalter V, Kiser R, Schneider D, Smedley J, Pung R, Gathuka M, Estes JD, Veazey RS, KewalRamani VN, Lifson JD, Bieniasz PD: A macaque model of HIV-1 infection. PNAS 106:4425-4429, 2009.
6. Hansen SG, Vieville C, Whizin N, Coyne-Johnson L, Siess DC, Drummond DD, Legasse AW, Axthelm MK, Oswald K, Trubey CM, Piatak MJr, Lifson JD, Nelson JA, Jarvis MA, Picker LJ: Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge. Nat Med 15:293-299, 2009.
7. Andersen H, Barsov EV, Trivett MT, Trubey CM, Giavedoni LD, Lifson JD, Ott DE, Ohlen C: Transduction with human telomerase reverse transcriptase immortalizes a rhesus macaque CD8+ T cell clone with maintenance of surface marker phenotype and function. AIDS Res Hum Retroviruses 23:456-465, 2007.
8. Wang D, Schmaljohn AL, Raja NU, Trubey CM, Juompan LY, Luo M, Deitz SB, Yu H, Woraratanadharm J, Holman DH, Moore KM, Swain BM, Pratt WD, Dong JY: De novo syntheses of Marburg virus antigens from adenovirus vectors induce potent humoral and cellular immune responses. Vaccine 24: 2975-2986, 2006.
9. Wang D, Hevey M, Juompan LY, Trubey CM, Raja NU, Deitz SB, Woraratanadharm J, Luo M, Yu H, Swain BM, Moore KM, Dong JY: Complex adenovirus-vectored vaccine protects guinea pigs fromthree strains of Marburg virus challenges. Virology 353(2): 324-332, 2006.
10. Wang D, Raja NU, Trubey CM, Juompan LY, Luo M, Woraratanadharm J, Deitz SB, Yu H, Swain BM, Moore KM, Pratt WD, Hart MK, Dong JY: Development of a cAdVax-based bivalent ebola virus vaccine that induces immune responses against both the Sudan and Zaire species of Ebola virus. J Virol 80: 2738-2746, 2006.
11. Chertova E, Chertov O, Coren LV, Roser JD, Trubey CM, Bess JW, Sowder RC, Barsov E, Hood BL, Fisher RJ, Nagashima K, Conrads TP, Veenstra TD, Lifson JD, Ott DE: Proteomic and biochemical analysis of purified HIV-1 produced from infected monocyte-derived macrophages. J Virol 80:9039-9052, 2006.
12. Trubey CM, Chertova E, Coren LV, Hilburn JM, Hixson CV, Nagashima K, Lifson JD, Ott DE: Quantitation of HLA class II protein incorporated into human immunodeficiency type 1 virions purified with anti-CD45 immunoaffinity depletion of microvesicles. J Virol 77:12699-12709, 2003.
13. Pinto LA, Grene E, Baker R, Trubey CM, Torres N, Trivett M, Shearer GM. Human immunodeficiency virus inhibitory activity generated by antigen-stimulated cord blood leukocytes. AIDS 17(9): 1389-1392, 2003.
14. Rugeles MT, Trubey CM, Bedoya VI, Pinto LA, Oppenheim JJ, Rybak SM, Shearer GM. Ribonuclease is partly responsible for the human immunodeficiency virus type 1 inhibitory effect activated by HLA alloantigen recognition. AIDS 17(4): 481-486, 2003.
15. Grene E, Pinto LA, Cohen SS, Trubey CM, Trivett MT, Simonis TB, Liewehr DJ, Steinberg SM, Shearer GM: Generation of alloantigen stimulated anti HIV activity is associated with HLA A*02 expression. J Infect Dis 183(3): 409-416, 2001.

Staffing

• Abigail B. Lara, Research Associate II
• James Thomas, Ph.D., Scientist I

Reagents available for non-commercial use:

SIV ENV monoclonal antibody (clone 7D3) labeled with Alexa Fluor 647 upon request by contacting Mr. Trubey