Frederick National Laboratory for Cancer Research
Building 535, Room 532
Frederick, MD 21702-1201
Mr. Mac Trubey received his B.S. in 1995 from the University of Maryland and his
M.S. degree in 2002 from Hood College. In 1993, he began his research career at
the University of Maryland Cancer Center, under Dr. Nicholas Bachur. In 1996, Mr.
Trubey began working at Frederick National Laboratory for Cancer Research, in an
HIV research laboratory under Dr. Gene Shearer and moved to the Retroviral Pathogenesis
Section of the AIDS and Cancer Virus Program in 2002, under Dr. Jeff Lifson. With
over 14 years of advanced flow cytometry and immunology experience, Mr. Trubey has
served as the Head of the Cellular Immunity Core, since its establishment in 2009.
The mission of the Cellular Immunity Core is to provide ACVP investigators and collaborators
with quantitative and multi-parametric cellular analysis and cell separation using
advanced flow cytometry methods and instrumentation.
The CIC is a centralized flow cytometry and cellular immunology resource for the
ACVP; providing instrumentation and essential services for NHP studies, investigators
and collaborators. The activities of the CIC include: providing state-of-the-art
phenotypic and functional immune monitoring for internal and collaborative NHP studies;
identifying cost-effective reagents and sourcing or producing custom reagents where
needed; providing cutting-edge flow cytometry support and data analysis to the ACVP
and collaborators; determining the needs of investigators and providing advice as
to the utility of flow cytometry for their specific research problems and goals;
providing technical expertise and training to ACVP investigators and staff in the
application of basic and advanced flow cytometry techniques; and developing innovations
to address the current and future flow cytometry needs of the ACVP.
Advanced instrumentation maintained by the CIC include: a 3-laser, 12-color Becton
Dickinson FACSAria II cell sorter equipped with an aerosol management system, sample
temperature control module, and a cell deposition unit for single-cell and plate/slide
sorting; a 4-laser, 16-color digital Becton Dickinson LSR-II analytical flow cytometer
equipped with a green laser and plate autosampler; and a 2-laser, 4-color analog
Becton Dickinson FACSCalibur flow cytometer equipped with a 40-tube carousel.