Leidos Biomedical Research, Inc.
Frederick National Laboratory for Cancer Research
Building 535, Room 532
Frederick, MD 21702-1201
Mr. Mac Trubey received his B.S. in 1995 from the University of MD and his M.S. degree in 2002 from Hood College. In 1993, he began his research career at the University of MD Cancer Center, under Dr. Nicholas Bachur. In 1996, Mr. Trubey began working at NCI-Frederick, in an HIV research laboratory under Dr. Gene Shearer and moved to the Retroviral Pathogenesis Section of the AIDS and Cancer Virus Program in 2002, under Dr. Jeff Lifson. With over 20 years of advanced flow cytometry and immunology experience, Mr. Trubey has served as the Head of the Cellular Immunity Core, since its establishment in 2009.
Mission: The mission of the Cellular Immunity Core is to provide ACVP investigators and collaborators with quantitative and multi-parametric cellular analysis and cell separation using advanced flow cytometry methods and instrumentation.
Key Enabling Technologies: Polychromatic flow cytometry, infectious cell sorting, advanced phenotypic and functional cellular immune monitoring, complex multi-parameter flow cytometry data analysis. Advanced instrumentation include: BD FACSAria II 3-laser, 12-color infectious cell sorter equipped with an aerosol management system, sample temperature control module, and a cell deposition unit for single-cell and plate/slide-based sorting; BD Fortessa X-20 (20-color) and LSR-II (16-color) 4-laser, digital flow cytometers equipped with high-sensitivity green lasers and 96-/384-well plate autosamplers; and a BD FACSVerse 3-laser, 8-color flow cytometer equipped with a 96-well plate/40-tube autosampler and a volumetric sensor for performing absolute cell counting.
Scope of Activities: The activities of the CIC include:
i) providing state-of-the-art phenotypic and functional immune monitoring for internal and collaborative non-human primate (NHP) studies
ii) providing cutting-edge flow cytometry support and data analysis to the ACVP and collaborators
iii) determining the needs of investigators and advise as to the utility of flow cytometry towards their specific research endeavors and problems
iv) providing technical expertise and training to ACVP investigators and staff in the application of basic and advanced flow cytometry techniques
v) developing innovations to address the current and future immunology and flow cytometry needs of the ACVP