Comprehensive support for studies of cell immunophenotyping, cell sorting, cell cycle, apoptosis and cell proliferation.
Flow cytometry (FCM) is a unique experimental technology, which provides rapid, quantitative, multi-parametric,
single cell analysis and separation. The mission of the flow cytometry core encompasses basic research support
provided to members of the Frederick-CCR community. The laboratory staff also participates in the development
of flow cytometry applications and resources for use in basic research. Resource development in the lab includes
advances in methodology and up-dating the instrumentation and computer/software. The core staff also trains investigators
to operate the analyzer instruments. This laboratory is contract-operated with oversight by a user based steering
Dedicated to CCR and Program
The CCR Flow Cytometry Core provides multi-color flow cytometry analysis of live or fixed single-cell suspensions
derived from various tissues and cell cultures. The group can offer up to 11 parameter cell surface markers coupled
with calcium-flux or cell cycle analysis. Standard and high-speed cell sorting of single-cell suspensions of up
to 11 colors is available in either bulk sorts or by single cell deposition in 96-well plates. Cell cycle analysis
software is available for cell ploidy, cell cycle, and apoptotic analysis of cell populations.
Part of the mission of the group is to give advice and experimental design suggestions to the investigators who
use this facility. This advice includes trouble-shooting when results do not meet the expectation of the investigator.
Staining protocols are kept on file and are provided to the investigator.