Comprehensive support for studies of cell immunophenotyping, cell sorting, cell cycle, apoptosis and cell proliferation.
Flow cytometry (FCM) is a unique experimental technology, which provides rapid, quantitative, multi-parametric,
single cell analysis and separation. The mission of the flow cytometry core encompasses basic research
provided to members of the Frederick-CCR community. The laboratory staff also participates in the
of flow cytometry applications and resources for use in basic research. Resource development in
the lab includes
advances in methodology and up-dating the instrumentation and computer/software. The core staff
also trains investigators
to operate the analyzer instruments. This laboratory is contract-operated with oversight by a user
Dedicated to CCR and Program
The CCR Flow Cytometry Core provides multi-color flow cytometry analysis of live or fixed single-cell
derived from various tissues and cell cultures. The group can offer up to 11 parameter cell surface
with calcium-flux or cell cycle analysis. Standard and high-speed cell sorting of single-cell suspensions
to 11 colors is available in either bulk sorts or by single cell deposition in 96-well plates. Cell
software is available for cell ploidy, cell cycle, and apoptotic analysis of cell populations.
Part of the mission of the group is to give advice and experimental design suggestions to the investigators
use this facility. This advice includes trouble-shooting when results do not meet the expectation
of the investigator.
Staining protocols are kept on file and are provided to the investigator.