||Dr. Kimberly Bishop-Lilly, NMRC
||High-throughput genotypic and phenotypic strategies for microbial identification and characterization: applications and limitations
- Frey, K.G., Herrera-Galeano, J.E., Redden, C.L., Luu, T.V., Servetas, S.L., Mateczun, A.J., Mokashi, V., and Bishop-Lilly, K.A.
Comparison of three next-generation sequencing platforms for metagenomic sequencing and
identification of pathogens in blood. BMC Genomics 2014 15:96.
- Ahmed, S., Awosika, J., Baldwin, C., Bishop-Lilly, K.A., et al.
Genomic comparison of Escherichia coli O104:H4 isolates from 2009 and 2011 reveals plasmid, and prophage heterogeneity, including shiga toxin encoding phage stx2. PLoS ONE 7(11):e48228.
- Chang, W.E., K. Sarver, B.W. Higgs, T.D. Read, N.M.E. Nolan, K.A. Bishop-Lilly, and S. Sozhamannan.
PheMaDB: A solution for storage, retrieval, and analysis of high throughput phenotypic data. BMC Bioinformatics 2011 12:109.
- Bishop-Lilly, K.A., M.J. Turrel, K.M. Willner, A. Butani, N.M.E. Nolan, S.M. Lentz, A. Akmal, T. Brahmbhatt, A. Mateczun, S. Sozhamannan, C.A.
Whitehouse, and T.D. Read. Arbovirus Detection in Insect Vectors by Rapid, High-Throughput Pyrosequencing. PLoS Negl Trop Dis 4(11): e878.
||Dr. Tommy Turbyville, NCI
||Using imaging technology to target an oncogene called Ras
||Dr. Kylie Walters, NCI
||Using structural biology to understanding protein degradation
||Dr. Reid Frederick, USDA
||Emerging Foreign Fungal Plant Pathogens and the Threat to U.S. Agriculture
- Increased foreign travel and global trade provide opportunities for exotic plant pathogens to enter the United States. If these newly introduced pathogens become established, costly epidemics may result. The USDA-ARS Foreign Disease-Weed Science Research Unit operates a unique Biosafety Level 3P (Plant) Containment Facility for conducting research on exotic plant pathogens that are of potential threat to U.S. agriculture. Our research focuses on identifying and characterizing foreign fungal plant pathogens, developing an understanding of their biology, genetics and epidemiology, and screening germplasm for resistant sources are key in preventing their entry and in reducing the damage that might result should they enter and become established in the U.S. Diseases currently under study include chrysanthemum white rust, gladiolus rust, soybean rust, red leaf blotch of soybeans, and wheat blast. The results of our research will benefit U.S. growers by facilitating greater preparedness against foreign fungal plant pathogens including an understanding of their biology, methods for their identification and detection, and knowledge of sources of crop resistance.
- Kingsolver, C. H., Melching, J. S., and Bromfield, K. R. 1983. The threat of exotic plant pathogens to agriculture in the United States. Plant Disease 67:595-600.
- Melching, J. S., Bromfield, K. R., and Kingsolver, C. H. 1983. The plant pathogen containment facility at Frederick, Maryland. Plant Disease 67:717-722.
- Palm, M. E. 2001. Systematics and the impact of invasive fungi on agriculture in the United States. Bioscience 51:141-147.
- Miles, M. R., Hartman, G. L., and Frederick, R. D. 2003. Soybean rust: Is the U.S. crop at risk? APSnet Features.
- Stokstad, E. 2004. Plant pathologists gear up for battle with dread fungus. Science 306:1672-1673.
||Dr. Todd Kijek, USAMRIID
||Francisella tularensis: virulence mechanisms and vaccine development
||Dr. Victoria Jensen, NBACC
BSL-4 High Risk Pathogens: Exploring the Spectrum
- Taylor SL, Wahl-Jensen V, Copeland AM, Jahrling PB, Schmaljohn CS.
Endothelial cell permeability during hantavirus infection involves factor XII-dependent increased activation of the kallikrein-kinin system.
PLoS Pathog. 2013;9(7):e1003470.
- Wahl-Jensen V, Bollinger L, Safronetz D, de Kok-Mercado F, Scott DP, Ebihara H.
Use of the Syrian hamster as a new model of ebola virus disease and other viral hemorrhagic fevers.
Viruses. 2012 Dec 14;4(12):3754-84.
- Cann JA, Jahrling PB, Hensley LE, Wahl-Jensen V.
Comparative pathology of smallpox and monkeypox in man and macaques.
J Comp Pathol. 2013 Jan;148(1):6-21.
- Hoenen T, Groseth A, de Kok-Mercado F, Kuhn JH, Wahl-Jensen V.
Minigenomes, transcription and replication competent virus-like particles and beyond: reverse genetics systems for filoviruses and other negative stranded hemorrhagic fever viruses.
Antiviral Res. 2011 Aug;91(2):195-208.
- Hooper JW, Ferro AM, Wahl-Jensen V.
Immune serum produced by DNA vaccination protects hamsters against lethal respiratory challenge with Andes virus.
J Virol. 2008 Feb;82(3):1332-8.
||Dr. Joel Bozue, USAMRIID
||Molecular pathogenesis of Bacillus anthracis and Yersinia pestis
- Bozue J, Cote CK, Webster W, Bassett A, Tobery S, Little S, Swietnicki W.
A Yersinia pestis YscN ATPase mutant functions as a live attenuated vaccine against bubonic plague in mice.
FEMS Microbiol Lett. 2012 Jul;332(2):113-21.
- Bozue J, Mou S, Moody KL, Cote CK, Trevino S, Fritz D, Worsham P.
The role of the phoPQ operon in the pathogenesis of the fully virulent CO92 strain of Yersinia pestis and the IP32953 strain of Yersinia pseudotuberculosis.
Microb Pathog. 2011 Jun;50(6):314-21.
- Bozue J, Moody KL, Cote CK, Stiles BG, Friedlander AM, Welkos SL, Hale ML.
Bacillus anthracis spores of the bclA mutant exhibit increased adherence to epithelial cells, fibroblasts, and endothelial cells but not to macrophages.
Infect Immun. 2007 Sep;75(9):4498-505.
||Dr. Ian Gut, NBACC
||Utilizing mouse embryonic stem cell derived neurons to investigate the mechanisms of toxin-mediated neuronal modulation
- Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM.
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
PLoS One. 2013 May 14;8(5):e64423.
- Hubbard KS, Gut IM, Lyman ME, Tuznik KM, Mesngon MT, McNutt PM.
High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology research.
BMC Neurosci. 2012 Oct 24;13:127.
- McNutt P, Celver J, Hamilton T, Mesngon M.
Embryonic stem cell-derived neurons are a novel, highly sensitive tissue culture platform for botulinum research.
Biochem Biophys Res Commun. 2011 Feb 4;405(1):85-90.
This seminar series is sponsored by the National Interagency Confederation for Biological
Research (NICBR). The NICBR includes the following agencies National Cancer Institute
at Frederick (NCI-F), United States Army Medical Research Institute of Infectious
Diseases (USAMRIID), Centers for Disease Control (CDC), US Department of Agriculture
Foreign Disease Weed Science Research Unit (USDA FDWSRU), US Department of Homeland
Security National Biodefense Analysis and Countermeasures Center (DHS NBACC), National
Institute of Allergy and Infectious Diseases Integrated Research Facility (NIAID
IRF), and the Navy Medical Biodefense Research Lab (NMBDRL). The series is open
to all NICBR students and employees. Students from outside the Frederick National Lab and
Ft. Detrick communities are also welcome.