Advanced Technology Program (ATP)

What’s New at the EML

News

	CONGRATULATIONS:  Thirty-eight-year electron microscopy veteran Kunio Nagashima was awarded the 2012 Outstanding Technologist (Biological Sciences) Award from the Microscopy Society of America (MSA), the world’s largest professional association of microscopists.  The award was given in recognition of the unique sample preparation techniques that Kunio has developed, including in-situ processing/embedding of cell cultures and a custom-designed way to ultracentrifuge small amounts of viruses into an embedding mold for further fixation and embedding.  Developing the in-situ embedding has been very important in successfully studying virus budding and cell-to-cell virus transmission.
	FNLCR SPRING RESEARCH FESTIVAL AWARD:  Ferri Soheilian, EML member supporting research for the Drug Resistance Program (DRP), was presented an award in recognition of an outstanding poster in the Structural Biology and Chemistry catagory for her poster: High-Pressure Freezing and Freeze-Substitution Shows Enhanced Details in Niemann-Pick Type C Disease Cells.

Publications

The objective of EML personnel is to assist NIH investigators achieve their research goals.  In accomplishing this, our research partners have included data generated by the EML in their publications.  Below are a few of the most recent publications that have resulted from our collaborative efforts.  Also check out our publication covers.

Bello NF, Dussupt V, Sette P, Rudd V, Nagashima K, Bibollet-Ruche F, Chen C, Montelaro RC, Hahn BH, Bouamr FJ:  Budding of Retroviruses Utilizing Divergent L domains Requires Nucleocapsid. J Virol, 2012.

Del Prete GQ, Kearney MF, Spindler J, Wiegand A, Chertova E, Roser JD, Estes JD, Hao XP, Trubey CM, Lara A, Lee K, Chaipan C, Bess JW Jr, Nagashima K, Keele BF, Pung R, Smedley J, Pathak VK, Kewalramani VN, Coffin JM, Lifson JD: Restricted Replication of Xenotropic Murine Leukemia Virus-Related Virus in Pigtailed Macaques. J. Virol. doi:10.1128/JVI.06886-11, 2012.

Hasumi H, Baba M, Hasumi Y, Huang Y, Oh H, Hughes RM, Klein ME, Takikita S, Nagashima K, Schmidt LS, Linehan WM:  Regulation of Mitochondrial Oxidative Metabolism by Tumor Suppressor FLCN.  J Natl Cancer Inst. 2012 Nov 12.

O’Carroll IP, Soheilian F, Kamata A, Nagashima, K, and Rein A:  Elements in HIV-1 Gag Contributing to Virus Particle Assembly. Virus Res, 2012.

O’Carroll IP, Crist RM, Mirro J, Harvin D, Soheilian F, Kamata A, Nagashima K, and Rein A: Functional Redundancy in HIV-1 Viral Particle Assembly. J. Virol. 86: 2012.

Olkhanud PB, Mughal M, Ayukawa K, Malchinkhuu E, Bodogai M, Feldman N, Rothman S, Lee JH, Chigurupati S, Okun E, Nagashima K, Mattson MP, Biragyn A:  DNA immunization with HBsAg-based particles expressing a B cell epitope of amyloid β-peptide attenuates disease progression and prolongs survival in a mouse model of Alzheimer’s disease. Vaccine, 2012.

Srivastava S, Kashiwaya Y, King MT, Baxa U, Tam J, Niu G, Chen X, Clarke K, Veech RL:  Mitochondrial biogenesis and increased uncoupling protein 1 in brown adipose tissue of mice fed a ketone ester diet. FASEB J, 2012.

Waki, K, Durell, SR, Soheilian, F, Nagashima, K, Butler, SL., and Freed, EO:  Structural and Functional Insights into the HIV-1 Maturation Inhibitor Binding Pocket.  PLoS Pathog. 8(11), 2012.

Clemens K, Larsen L, Zhang M, Kuznetsov Y, Bilanchone V, Randall A, Harned A, Dasilva R, Nagashima K, McPherson A, Baldi P, Sandmeyer S:  The TY3 Gag3 Spacer Controls Intracellular Condensation and Uncoating. J Virol, 85(7):3055-66, 2011.

Datta SA, Temeselew LG, Crist RM, Soheilian F, Kamata A, Mirro J, Harvin D, Nagashima K, Cachau RE, Rein A:  On the Role of the SP1 Domain in HIV-1 Particle Assembly: A Molecular Switch? J Virol, 2011.

Joshi A, Garg H, Ablan S, Freed EO, Nagashima K, Manjunath N, Shankar P:  Targeting the HIV entry, assembly and release pathways for anti-HIV gene therapy. Virology, 415(2):95-106, 2011.

Mizuno N, Baxa U, Steven AC:  Structural dependence of HET-s amyloid fibril infectivity assessed by cryoelectron microscopy. Proc Natl Acad Sci USA, 108(8):3252-7, 2011.

Smith B, Lyakhov I, Loomis K, Needle D, Baxa U, Yavlovich A, Capala J, Blumenthal R, Puri A:  Hyperthermia-triggered intracellular delivery of anticancer agent to HER2(+) cells by HER2-specific affibody (ZHER2-GS-Cys)-conjugated thermosensitive liposomes (HER2(+) affisomes).  J Control Release, 153(2):187-94, 2011.

Yoko Shiba, Ruibai Luo, Jenny E Hinshaw, Tomasz Szul, Ryo Hayashi, Elizabeth Sztul, Kunio Nagashima, Ulrich Baxa, and Paul A Randazzo:  ArfGAP1 promotes COPI vesicle formation by facilitating coatomer polymerization. Cell Logist, 1(4): 139–154, 2011.