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Calcium Assays

What it does best:

What it can’t do:

Examples of uses:

General hints and tips:

Dyes: Cameleon [FRET], Fluo-3, Fura-2

Websites/Reference Papers:

The Molecular Probes website in chapter 19, section 19.3 discusses how to measure Calcium ions using the ratio of two dyes.

Ratiometric confocal Ca(2+)-measurements with visible wavelength indicators in isolated cardiac myocytes. Lipp P, Niggli E, Cell Calcium 1993; (14):5 359-372.

Page last updated November 2, 2010 @ 2:31 pm